, Volume 19, Issue 4-5, pp 287-293

Functions of lumican and fibromodulin: Lessons from knockout mice

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Abstract

Lumican and fibromodulin are collagen-binding leucine-rich proteoglycans widely distributed in interstitial connective tissues. The phenotypes of lumican-null (Lum −/−), Fibromodulin-null (Fmod −/−) and compound double-null (Lum −/− Fmod −/−) mice identify a broad range of tissues where these two proteoglycans have overlapping and unique roles in modulating the extracellular matrix and cellular behavior. The lumican-deficient mice have reduced corneal transparency and skin fragility. The Lum −/− Fmod −/− mice are smaller than their wildtype littermates, display gait abnormality, joint laxity and age-dependent osteoarthritis. Misaligned knee patella, severe knee dysmorphogenesis and extreme tendon weakness are the likely cause for joint-laxity. Fibromodulin deficiency alone leads to significant reduction in tendon stiffness in the Lum +/+ Fmod −/− mice, with further loss in stiffness in a lumican gene dose-dependent way. At the level of ultrastructure, the Lum −/− cornea, skin and tendon show irregular collagen fibril contours and increased fibril diameter. The Fmod −/− tendon contains irregular contoured collagen fibrils, with increased frequency of small diameter fibrils. The tendons of Lum −/− Fmod −/− have an abnormally high frequency of small and large diameter fibrils indicating a de-regulation of collagen fibril formation and maturation. In tissues like the tendon, where both proteoglycans are present, fibromodulin may be required early in collagen fibrillogenesis to stabilize small-diameter fibril-intermediates and lumican may be needed at a later stage, primarily to limit lateral growth of fibrils Published in 2003.