Biotechnology Letters

, Volume 25, Issue 17, pp 1473–1477

Immobilized laccase for decolourization of Reactive Black 5 dyeing effluent


  • Andrea Zille
    • Department of Textile EngineeringUniversity of Minho
  • Tzanko Tzanov
    • Department of Textile EngineeringUniversity of Minho
  • Georg M. Gübitz
    • Department of Environmental BiotechnologyGraz University of Technology
    • Department of Textile EngineeringUniversity of Minho

DOI: 10.1023/A:1025032323517

Cite this article as:
Zille, A., Tzanov, T., Gübitz, G.M. et al. Biotechnology Letters (2003) 25: 1473. doi:10.1023/A:1025032323517


Reactive Black 5 industrial dyeing effluent was decolourized by free and immobilized laccase. The stability of the enzyme (194 h free and 79 h immobilized) depended on the dyeing liquor composition and the chemical structure of the dye. In the decolourization experiments with immobilized laccase, two phenomenons were observed – decolourization due to adsorption on the support (79%) and dye degradation due to the enzyme action (4%). Dyeing in the enzymatically recycled effluent provided consistency of the colour with both bright and dark dyes.

decolourizationlaccaserecycling of dyeing effluentsstability
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© Kluwer Academic Publishers 2003