Article

Biotechnology Letters

, Volume 25, Issue 17, pp 1473-1477

First online:

Immobilized laccase for decolourization of Reactive Black 5 dyeing effluent

  • Andrea ZilleAffiliated withDepartment of Textile Engineering, University of Minho
  • , Tzanko TzanovAffiliated withDepartment of Textile Engineering, University of Minho
  • , Georg M. GübitzAffiliated withDepartment of Environmental Biotechnology, Graz University of Technology
  • , Artur Cavaco-PauloAffiliated withDepartment of Textile Engineering, University of Minho Email author 

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Abstract

Reactive Black 5 industrial dyeing effluent was decolourized by free and immobilized laccase. The stability of the enzyme (194 h free and 79 h immobilized) depended on the dyeing liquor composition and the chemical structure of the dye. In the decolourization experiments with immobilized laccase, two phenomenons were observed – decolourization due to adsorption on the support (79%) and dye degradation due to the enzyme action (4%). Dyeing in the enzymatically recycled effluent provided consistency of the colour with both bright and dark dyes.

decolourization laccase recycling of dyeing effluents stability