Genetic relationships among species and cultivars of Pistacia using RAPDs and AFLPs
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The genus Pistacia (Anacardiaceae) includes 11 species divided into four sections, according to leaf characters and nut morphology. Recently two monophyletic groups have been proposed by using cpDNA,Lentiscus and Terebinthus,containing evergreen and deciduous species, respectively. In the present work molecular markers, derived from two different methods, RAPD and AFLP, are used to study the relationships of native and introducedPistacia species present in Greece. According to the cophenetic correlation coefficients best results for both methods were obtained by using the Jaccard algorithm and the UPGMA clustering method. However, phenograms were constructed using the NJ method (rcs= 0.987 for RAPDs and rcs= 0.982 for AFLP), since it is less sensitive against varying mutation rates. Correlation among the genetic similarity (GS) matrices for the two methods was high(r = 0.941). The AFLP and RAPD phenograms were comparable with minor clustering differences. According to our results, two main branches are obtained, one containing the evergreen species P. lentiscusand the resin producing P. lentiscuscv. Chia (cultivated only in the island of Chios), and the other branch containing the deciduous species P. terebinthus,P. palaestina and P. vera, P. chinensis was clustered either with the evergreen species (RAPD data) or with the deciduous species (AFLP data). P. palaestina is clustered to P. terebinthus and can be considered as a subspecies of P. terebinthus, since its GS values are close or smaller than GS values of entries within species (P. vera). The four female cultivars were found to have a narrow genetic basis, probably related to cultivar ‘Nazareth‘ with Syrian origin.
- Barker, J.H.A., M. Matthes, G.M. Arnold, K.J. Edwards, I. Ahman, S. Larsson & A. Karp, 1999. Characterization of genetic diversity in potential biomass willows (Salix spp.) by RAPD and AFLP analyses. Genome 42: 173-183. CrossRef
- Cervera, M.-T., J.A. Cabezas, J.C. Sancha, F. Martinez de Toda & J.M. Martinez-Zapater, 1998. Applications of AFLPs to the characterization of grapevine Vitis vinifera L. genetic resources. A case study with accessions from Rioja (Spain). Theor Appl Genet 97: 51-59. CrossRef
- El-Oqlah, A.A., 1996. Biosystematic research on the genus Pistacia in Jordan. In: S. Padulosi, T. Caruso & E. Barone (Eds.), Taxonomy, Distribution, Conservation and Uses of Pistacia Genetic Resources, pp. 12-19. IPGRI, Rome.
- Engler, A., 1936. Syllabus der Pflanzenfamilien. Berlin. Germany.
- Garcia-Mas, J., M. Oliver, H. Gomez-Paniagua & M.C. de Vicente, 2000. Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon. Theor Appl Genet 101: 860-864. CrossRef
- Hormaza, J.I., L. Dollo & V.S. Polito, 1994. Determination of relatedness and geographical movements of Pistacia vera L. (pistachio; Anacardiaceae) germplasm by RAPD analysis. Econ Bot 48: 349-358.
- Hormaza, J.I., K. Pinney & V.S. Polito, 1998. Genetic diversity of pistachio (Pistacia vera, Anacardiaceae) germplasm based on Randomly Amplified Polymorphic DNA (RAPD) markers. Econ Bot 52: 78-87.
- Jones, C.J., K.J. Edwards, S. Castiglione, M.O. Winfield, F. Sala, C. Van der Wiel, B.L. Vosman, M. Matthes, A. Daly, R. Brettschneider, P. Bettini, M. Buiatti, E. Maestri, N. Marmiroli, R.L. Aert, G. Volckaert, J. Rueda, A. Vazquez & A. Karp, 1998a. Reproducibility testing of RAPDs by a network of European laboratories. In: A. Karp, P.G. Isaac & D.S. Ingram (Eds.), Molecular Tools for Screening Biodiversity: Plants and Animals, pp. 176-179. Chapman & Hall, London.
- Jones, C.J., K.J. Edwards, S. Castiglione, M.O. Winfield, F. Sala, C. Van der Wiel, B.L. Vosman, M. Matthes, A. Daly, R. Brettschneider, E. Maestri, N. Marmiroli, R.L. Aert, G. Volckaert & A. Karp, 1998b. Reproducibility testing of AFLPs by a network of European laboratories. In: A. Karp, P.G. Isaac & D.S. Ingram (Eds.), Molecular Tools for Screening Biodiversity: Plants and Animals, pp. 191-192. Chapman & Hall, London.
- Kafkas, S. & R. Perl-Treves, 2001. Morphological and molecular phylogeny of Pistacia species in Turkey. Theor Appl Genet 102: 908-915. CrossRef
- Karp, A., O. Seberg & M. Buiatti, 1996. Molecular techniques in the assessment of botanical diversity. Ann Bot 78: 143-149. CrossRef
- Loukas, M. & C.A. Pontikis, 1979. Pollen isozyme polymorphism in types of Pistacia vera and related species as an aid in taxonomy. J Hort Sci 54: 95-102.
- Monte-Corvo, L., L. Cabrita, C. Oliveira & J. Leitao, 2000. Assessment of genetic relationships among Pyrus species and cultivars using AFLP and RAPD markers. Genet Res Crop Evol 47: 257-265. CrossRef
- Parfitt, D.E. & M.L. Badenes, 1997. Phylogeny of the genus Pistacia as determined from analysis of the chloroplast genome. Proc Natl Acad Sci USA 94: 7987-7992. CrossRef
- Patzak, J., 2001. Comparison of RAPD, STS, ISSR and AFLP molecular methods used for assessment of genetic diversity in hop (Humulus lupulus L.). Euphytica 121: 9-18. CrossRef
- Rohlf, M., 1998. NTSYSpc. Numerical Taxonomy and Multivariate Analysis System. Version 2.02i. Department of Ecology and Evolution. State University of New York.
- Savidis, T., 2000. The mastic tree of Chios (Pistacia lentiscus var. Chia). Kyriakides Bros, Thessaloniki-Greece (in Greek).
- Sneath, P.H. & R.R. Sokal, 1973. Numerical Taxonomy: The Principles and Practice of Numerical Classification. W.H. Freeman and Company, San Francisco.
- Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper & M. Zabeau, 1995. AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23: 4407-4414.
- Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18: 6531-6535.
- Zohary, D., 1996. The genus Pistacia L. In: S. Padulosi, T. Caruso & E. Barone (Eds.), Taxonomy, Distribution, Conservation and Uses of Pistacia Genetic Resources, pp. 1-11. IPGRI, Rome.
- Zohary, M., 1952. A monographic study of the genus Pistacia. Palestine J Bot Jerusalem Ser 5: 187-228.
- Genetic relationships among species and cultivars of Pistacia using RAPDs and AFLPs
Volume 132, Issue 3 , pp 279-286
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- 1. Department of Genetics Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece
- 2. Pomology Laboratory, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece