Biotechnology Letters

, Volume 25, Issue 15, pp 1281–1285

Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris

Authors

  • Philippe Baumgartner
    • Quality, Health & Nutrition GroupScottish Crop Research Institute
  • Karen Harper
    • Quality, Health & Nutrition GroupScottish Crop Research Institute
  • Romaan J.M. Raemaekers
    • Department Agricultural and Environmental Sciences, King George VI BuildingUniversity of Newcastle
  • Alain Durieux
    • Unité de BiotechnologieInstitut Meurice
  • Angharad M.R. Gatehouse
    • Department Agricultural and Environmental Sciences, King George VI BuildingUniversity of Newcastle
  • Howard V. Davies
    • Quality, Health & Nutrition GroupScottish Crop Research Institute
    • Quality, Health & Nutrition GroupScottish Crop Research Institute
Article

DOI: 10.1023/A:1025007901322

Cite this article as:
Baumgartner, P., Harper, K., Raemaekers, R.J. et al. Biotechnology Letters (2003) 25: 1281. doi:10.1023/A:1025007901322

Abstract

The gene coding for agglutinin from Galanthus nivalis (GNA) was expressed in, and secreted by, the methylotrophic yeast, Pichia pastoris. Transformants of P. pastoris were selected and a process to produce and purify gram quantities of recombinant GNA was developed. GNA was secreted at approximately 80 mg l−1 at the 200 l scale and was purified to 95% homogeneity using hydrophobic interaction chromatography. The recombinant protein was similar to the protein synthesised in plant with respect to structure and biological activity.

fed-batch cultureGalanthus nivalis agglutininlarge-scale purificationlectinPichia pastoris

Copyright information

© Kluwer Academic Publishers 2003