Journal of Neuro-Oncology

, Volume 64, Issue 1, pp 31–44

Robust Ability of IFN-γ to Upregulate Class II MHC Antigen Expression in Tumor Bearing Rat Brains

  • Tanya Dutta
  • Alexander Spence
  • Lois A. Lampson
Article

DOI: 10.1023/A:1024973506989

Cite this article as:
Dutta, T., Spence, A. & Lampson, L.A. J Neurooncol (2003) 64: 31. doi:10.1023/A:1024973506989

Abstract

T cells are attractive for delivering therapy to brain tumor, especially disseminated micro-tumor. However, to trigger effector function, tumor antigen must be re-presented to T cells, via major histocompatibility complex (MHC) proteins, at the tumor site. In normal brain, MHC+ antigen-presenting cells (APC) are rare, but abundant after gamma interferon (IFN-γ) injection. Here we studied tumor-bearing brains. IFN-γ (or buffer) was injected stereotactically into brains with established tumors from a panel of immunologically varied glioma cell lines, some expressing b-galactosidase as a micro-tumor marker. Four days later, cryostat sections were stained for tumor and MHC proteins. In phosphate-buffered saline-injected controls, class II MHC+ potential APC (microglia, macrophages) were seen only at (some) tumor sites. In rats that received IFN-γ, class II+ potential APC were widespread, including all actual and potential micro-tumor sites and all tumor-free areas. In the same slides, neither class I nor class II MHC antigen was detected in neural cells or most tumor cells. This MHC pattern favors indirect re-presentation of tumor antigen, by tumor-adjacent APC. The robust response to IFN-γ might also be exploited in other ways: activated microglia and macrophages can attack tumor directly, and class II+ APC may help mark micro-tumor sites.

brain neoplasms class II major histocompatibility complex (MHC) gamma interferon (IFN-γ) glioma immunotherapy macrophages microglia 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Tanya Dutta
    • 1
  • Alexander Spence
    • 2
  • Lois A. Lampson
    • 1
  1. 1.Brain Tumor Immunology Laboratory, Department of Neurosurgery, Brigham and Women's Hospital and Harvard Medical SchoolCNS &Boston
  2. 2.Department of NeurologyUniversity of WashingtonSeattleUSA

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