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Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates

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Abstract

The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.

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References

  • Allen S, Davies J, Dawkes AC, Davies MC, Edwards JC, Parker MC, Roberts CJ, Sefton J, Tendler SJB, Williams PM (1996) In situ observation of streptavidin-biotin binding on an immunoassay well surface using an atomic force microscope. FEBS Lett. 390: 161–164.

    Google Scholar 

  • Al-Harthi L, Marchetti G, Steffens CM, Poulin JF, Sekaly RP, Landay A (2000) Detection of T cell receptor circles (TRECs) as biomarkers for de novo T cell synthesis using a quantitative polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA). J. Immunol. Meth. 237: 187–197.

    Google Scholar 

  • Barbany G, Hagberg A, Waldenstrom E, Landegren U (1999) Molecular genetic applications of streptavidin-coated manifold supports. Biomol. Eng. 16: 105–111.

    Google Scholar 

  • Belotserkovskii BP, Johnston BH (1997) Denaturation and association of DNA sequences by certain polypropylene surfaces. Anal. Biochem. 251: 251–262.

    Google Scholar 

  • Belotserkovskii BP, Johnston BH, Gaillard C, Strauss F (1996) Polypropylene tube surfaces may induce denaturation and multimerisation of DNA. Science 271: 222.

    Google Scholar 

  • Bonen MR, Garcia AA, Hoffman SA (2001) A comparison of silver ion to streptavidin coated microplates. J. Microbiol. Meth. 44: 113–120.

    Google Scholar 

  • Cordiano I, Steffan A, Randi ML, Pradella P, Girolama A, Fabris F (1995) Biotin-avidin immobilisation of platelet glycoproteins (BAIPG): a new capture assay for the detection of anti-platelet antibodies. J. Immunol. Meth. 178: 121–130.

    Google Scholar 

  • Davies J, Roberts CJ, Dawkes A, Sefton J, Edwards JC, Glasbey TO, Haymes AG, Davies MC, Jackson DE, Lomas M, Shakesheff KM, Tendler SJB, Wilkins MJ, Williams PM (1994a) Use of scanning probe microscopy and surface plasmon resonance as analytical tools in the study of antibody-coated microtitre wells. Langmuir 10: 2654–2661.

    Google Scholar 

  • Davies J, Dawkes AC, Haymes AG, Roberts CJ, Sunderland RF, Wilkins MJ, Davies MC, Tendler SJB, Jackson DE, Edwards JC (1994b) A scanning tunnel microscopy comparison of passive antibody adsorption and biotinylated antibody linkage to streptavidin on microtitre wells. J. Immunol. Meth. 167: 263–269.

    Google Scholar 

  • Dorgan L, Magnotti R, Hou J, Engle T, Ruley K, Shull B (1999) Methods to determine biotin-binding capacity of streptavidincoated magnetic particles. J. Magnet. Magnet. Mat. 194: 69–75.

    Google Scholar 

  • Douglas AS, Monteith CA (1994) Improvements to immunoassays by covalent binding assay plates. Clin. Chem. 40: 1833–1837.

    Google Scholar 

  • Greening GE, Woodfield L, Lewis GD (1999) RT-PCR and chemiluminescent ELISA for detection of enteroviruses. J. Virol. Meth. 82: 157–166.

    Google Scholar 

  • Inman-Bamber J, Wan C, Garam T, Vohra R, van Daal A, Timms P (2002) Novel PCR-EIA method for the detection of Chlamydia pneumoniae in respiratory specimens. Mol. Cell. Probes 16: 57–61.

    Google Scholar 

  • Ito S, Yamazaki S, Kano K, Ikeda T (2000) Highly sensitive electrochemical detection of alkaline phosphatase. Anal. Chim. Acta. 424: 57–63.

    Google Scholar 

  • Lehel C, Daniel-Issakani S, Brasseur M, Strulovici B (1997) A chemiluminescent microtitre plate assay for sensitive detection of protein kinase activity. Anal. Biochem. 244: 340–346.

    Google Scholar 

  • Manzano M, Cocolin L, Cantoni C, Comi G (1998) Detection and identification of Listeria monocytogenes in food by PCR and oligonucleotide-specific capture plate hybridisation. Food Microbiol. 15: 651–657.

    Google Scholar 

  • Stevens J, Yu FS, Hassoun PM, Lanzillo JJ (1996) Quantification of polymerase chain reaction products: enzyme immunoassay based systems for digoxigenin-and biotin-labelled products that quantify either total or specific amplicons. Mol. Cell. Probes 10: 31–41.

    Google Scholar 

  • Wilchek M, Bayer EA (1990) Application of avidin-biotin technology. Meth. Enzymol. 184: 14–45.

    Google Scholar 

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Authors and Affiliations

  1. ABgene, ABgene House, Blenheim Road, Epsom, Surrey, KT19 9AP, UK

    Anne M. van der Valk, David N. Howbrook, Meg C. O'Shaughnessy & Ariel Louwrier

  2. School of Pharmacy and Biomolecular Sciences, University of Brighton, Moulsecoomb, Brighton, BN2 4GJ, UK

    Anne M. van der Valk, David N. Howbrook, Dipak K. Sarker & Andrew W. Lloyd

  3. School of Biological and Chemical Sciences, Birkbeck University of London, Malet Street, Bloomsbury, London, WC1E 7HX, UK

    Simon C. Baker

Authors
  1. Anne M. van der Valk
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  2. David N. Howbrook
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  3. Meg C. O'Shaughnessy
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  4. Dipak K. Sarker
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  5. Simon C. Baker
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  6. Ariel Louwrier
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  7. Andrew W. Lloyd
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Correspondence to Andrew W. Lloyd.

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van der Valk, A.M., Howbrook, D.N., O'Shaughnessy, M.C. et al. Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates. Biotechnology Letters 25, 1325–1328 (2003). https://doi.org/10.1023/A:1024951322955

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  • DOI: https://doi.org/10.1023/A:1024951322955

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