Photosynthesis Research

, Volume 76, Issue 1, pp 35–52

Mapping the carbon reduction cycle: a personal retrospective

  • James A. Bassham

DOI: 10.1023/A:1024929725022

Cite this article as:
Bassham, J.A. Photosynthesis Research (2003) 76: 35. doi:10.1023/A:1024929725022


The photosynthetic carbon reduction cycle was elucidated through the use of 14CO2 during photosynthesis to label metabolic intermediates. Mapping and proof of the cycle required identification of labeled metabolites, observation of changes in levels of labeled metabolites during transitions from light to dark and from high to low CO2 levels, determination of intramolecular distribution of 14C within the metabolites after a few seconds of photosynthesis with 14CO2, and estimation of metabolite concentrations, used to calculate true free energy changes at each step in the cycle.

James BasshamAndrew A. BensonMelvin Calvincarbon fixationenergeticsPeter Massiniphotosynthesisphotosynthetic carbon reduction (PCR) cycleradiocarbonsteady-statetransientsAlexander T. Wilson

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • James A. Bassham
    • 1
  1. 1.El CerritoUSA (