Plant Molecular Biology

, Volume 52, Issue 3, pp 537–551

Rice gene expression in response to N-acetylchitooligosaccharide elicitor: comprehensive analysis by DNA microarray with randomly selected ESTs

  • Chiharu Akimoto-Tomiyama
  • Katsumi Sakata
  • Junshi Yazaki
  • Keiko Nakamura
  • Fumiko Fujii
  • Kanako Shimbo
  • Kimiko Yamamoto
  • Takuji Sasaki
  • Naoki Kishimoto
  • Shoshi Kikuchi
  • Naoto Shibuya
  • Eiichi Minami
Article

DOI: 10.1023/A:1024890601888

Cite this article as:
Akimoto-Tomiyama, C., Sakata, K., Yazaki, J. et al. Plant Mol Biol (2003) 52: 537. doi:10.1023/A:1024890601888

Abstract

N-acetylchitooligosaccharides are potent elicitors to suspension-cultured rice cells, inducing a set of defense reactions. Expression of defense-related genes is considered to play an important role in defense reactions, and we employed microarray analysis of 8987 randomly selected expressed sequence tags to analyze the changes in gene expression caused by N-acetylchitooctaose. In this experiment, 166 genes were significantly induced and 93 genes were repressed. RNA gel blot analysis of 16 of these genes confirmed the microarray results. Of the 259 ESTs identified as responsive to N-acetylchytooctaose, 18 genes are related to signal transduction, including five calcium-dependent protein kinases (CDPKs). Among these, three novel CDPKs responsive to N-acetylchitooctaose were isolated.

CDPKmicroarrayN-acetylchitooligosaccharidesOryza sativarice cells

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Chiharu Akimoto-Tomiyama
    • 1
  • Katsumi Sakata
    • 2
  • Junshi Yazaki
    • 3
  • Keiko Nakamura
    • 4
  • Fumiko Fujii
    • 4
  • Kanako Shimbo
    • 4
  • Kimiko Yamamoto
    • 4
  • Takuji Sasaki
    • 2
  • Naoki Kishimoto
    • 3
  • Shoshi Kikuchi
    • 3
  • Naoto Shibuya
    • 1
  • Eiichi Minami
    • 1
  1. 1.Department of BiochemistryJapan
  2. 2.Department of Genome ResearchJapan
  3. 3.Department of Molecular GeneticsNational Institute of Agrobiological SciencesTsukubaJapan
  4. 4.STAFF InstituteKamiyokoba, TsukubaJapan