European Journal of Plant Pathology

, Volume 109, Issue 6, pp 589–597

Detection and Quantification of Spongospora subterranea in Soil, Water and Plant Tissue Samples Using Real-Time PCR

  • Pieter van de Graaf
  • Alison K. Lees
  • Danny W. Cullen
  • James M. Duncan
Article

DOI: 10.1023/A:1024764432164

Cite this article as:
van de Graaf, P., Lees, A.K., Cullen, D.W. et al. European Journal of Plant Pathology (2003) 109: 589. doi:10.1023/A:1024764432164

Abstract

A sensitive real-time polymerase chain reaction (PCR) assay was developed for the quantification of Spongospora subterranea, the cause of powdery scab and root galling in potato, and the vector of Potato mop top virus. A specific primer pair and a fluorogenic TaqMan® probe were designed to perform a quantitative assay for the detection of S. subterranea in soil, water and plant tissue samples. The assay was tested using DNA from cystosori, zoospores, plasmodia and zoosporangia of the pathogen. DNA was extracted directly from cystosori suspended in water and from clay soil with varying levels of added cystosori. DNA obtained from zoospores released into nutrient solution by cystosori in the presence of tomato bait plants was also tested, as was DNA from plasmodia and zoosporangia in infected tomato roots. In many cases, detection was successful even at low inoculum levels. This specific quantitative assay could therefore be a useful tool for studying the biology of S. subterranea, and for the optimisation of disease avoidance and control measures.

bait plantsinternal transcribed spacer regionsplasmodiophoridspowdery scabPotato mop top virusTaqMan®

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Pieter van de Graaf
    • 1
  • Alison K. Lees
    • 1
  • Danny W. Cullen
    • 1
  • James M. Duncan
    • 1
  1. 1.Scottish Crop Research InstituteInvergowrie, DundeeUK