Journal of Biological Physics

, Volume 29, Issue 2, pp 275–286

Synchrotron-Based FTIR Spectromicroscopy: Cytotoxicity and Heating Considerations

  • H.-Y.N. Holman
  • M.C. Martin
  • W.R. McKinney
Article

DOI: 10.1023/A:1024465414395

Cite this article as:
Holman, HY., Martin, M. & McKinney, W. Journal of Biological Physics (2003) 29: 275. doi:10.1023/A:1024465414395

Abstract

Synchrotron radiation-based Fouriertransform infrared (SR-FTIR)spectromicroscopy is a newly emergingbioanalytical and imaging tool. This uniquetechnique provides mid-infrared (IR)spectra, hence chemical information, withhigh signal-to-noise at spatial resolutionsas fine as 3 to 10 microns. Thus it enablesresearchers to locate, identify, and trackspecific chemical events within anindividual living mammalian cell. Mid-IRphotons are too low in energy (0.05–0.5eV) to either break bonds or to causeionization. In this review, we show thatthe synchrotron IR beam has no detectableeffects on the short- and long-termviability, reproductive integrity,cell-cycle progression, and mitochondrialmetabolism in living human cells, andproduces only minimal sample heating (<0.5°C). These studies haveestablished an important foundation forSR-FTIR spectromicroscopy in biological andbiomedical research.

Alcian blue BrdU colony formation cytotoxicity FTIR heating MTT spectromicroscopy synchrotron viability 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • H.-Y.N. Holman
    • 1
  • M.C. Martin
    • 1
  • W.R. McKinney
    • 1
  1. 1.Lawrence Berkeley National LaboratoryBerkeleyUSA

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