Biotechnology Letters

, Volume 25, Issue 13, pp 1007–1011

Resonant Raman quantification of zeaxanthin production from Flavobacterium multivorum

  • Prakash Bhosale
  • Igor V. Ermakov
  • Maia R. Ermakova
  • Werner Gellermann
  • Paul S. Bernstein
Article

DOI: 10.1023/A:1024199213519

Cite this article as:
Bhosale, P., Ermakov, I.V., Ermakova, M.R. et al. Biotechnology Letters (2003) 25: 1007. doi:10.1023/A:1024199213519

Abstract

Resonant Raman scattering was used as a novel, rapid, non-destructive optical technique to measure zeaxanthin levels in Flavobacterium multivorum ATCC 55238. Culture broth, after bacterial growth for 40 h, exhibited characteristic resonance Raman vibrational modes at 1159 cm−1 (C-C stretch) and 1525 cm−1 (C=C stretch) upon excitation at 488 nm. A striking correlation was observed between the carotenoid level as estimated by HPLC and by resonance Raman spectroscopy.

Flavobacterium multivorumRaman spectroscopyzeaxanthin

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Prakash Bhosale
    • 1
  • Igor V. Ermakov
    • 2
  • Maia R. Ermakova
    • 2
  • Werner Gellermann
    • 2
  • Paul S. Bernstein
    • 1
  1. 1.Department of Ophthalmology and Visual SciencesUniversity of Utah School of MedicineSalt Lake CityUSA
  2. 2.Department of Physics and Dixon Laser InstituteUniversity of UtahSalt Lake CityUSA