Journal of Biomolecular NMR

, Volume 26, Issue 4, pp 335–344

IBIS – A tool for automated sequential assignment of protein spectra from triple resonance experiments

  • Sven G. Hyberts
  • Gerhard Wagner
Article

DOI: 10.1023/A:1024078926886

Cite this article as:
Hyberts, S.G. & Wagner, G. J Biomol NMR (2003) 26: 335. doi:10.1023/A:1024078926886

Abstract

We have developed a tool for computer-assisted assignments of protein NMR spectra from triple resonance data. The program is designed to resemble established manual assignment procedures as closely as possible. IBIS exports its results in XEASY format. Thus, using IBIS the operator has continuous visual and accounting control over the progress of the assignment procedure. IBIS achieves complete assignments for those residues that exhibit sequential triple resonance connectivities within a few hours or days.

automated assignmentsbackbone assignmentsNMRprotein structuretriple resonance experiments

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Sven G. Hyberts
    • 1
  • Gerhard Wagner
    • 1
  1. 1.Department of Biological Chemistry and Molecular PharmacologyHarvard Medical SchoolBostonU.S.A