Biotechnology Letters

, Volume 25, Issue 11, pp 895–899

Laser-assisted microinjection into targeted animal cells

  • Samarendra K. Mohanty
  • Mrinalini Sharma
  • Pradeep K. Gupta
Article

DOI: 10.1023/A:1024038609045

Cite this article as:
Mohanty, S.K., Sharma, M. & Gupta, P.K. Biotechnology Letters (2003) 25: 895. doi:10.1023/A:1024038609045

Abstract

A pulsed (17 nanoseconds) Nd:YAG laser (1064 nm) was used to inject impermeable dyes (propidium iodide andiodide and merocyanine 540) and a plasmid (pEGFP-N1) encoding green fluorescent protein (GFP) into human breast adenocarcinoma cells (MCF-7). The cell membrane integrity and viability were fully preserved in this laser-assisted transfer.

microinjectionoptoporationphotodynamic treatmenttransfection

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Samarendra K. Mohanty
    • 1
  • Mrinalini Sharma
    • 1
  • Pradeep K. Gupta
    • 1
  1. 1.Biomedical Applications Section Center for Advanced TechnologyIndoreIndia