Laser-assisted microinjection into targeted animal cells
- Cite this article as:
- Mohanty, S.K., Sharma, M. & Gupta, P.K. Biotechnology Letters (2003) 25: 895. doi:10.1023/A:1024038609045
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A pulsed (17 nanoseconds) Nd:YAG laser (1064 nm) was used to inject impermeable dyes (propidium iodide andiodide and merocyanine 540) and a plasmid (pEGFP-N1) encoding green fluorescent protein (GFP) into human breast adenocarcinoma cells (MCF-7). The cell membrane integrity and viability were fully preserved in this laser-assisted transfer.