Biotechnology Letters

, Volume 25, Issue 11, pp 877–882

Application of statistically-based experimental designs for the optimization of nisin production from whey

  • Chuanbin Liu
  • Yan Liu
  • Wei Liao
  • Zhiyou Wen
  • Shulin Chen
Article

DOI: 10.1023/A:1024009027255

Cite this article as:
Liu, C., Liu, Y., Liao, W. et al. Biotechnology Letters (2003) 25: 877. doi:10.1023/A:1024009027255

Abstract

Statistically-based experimental designs were applied for the optimization of nisin production by Lactococcus lactis in a whey-based medium. Yeast extract, KH2PO4, and MgSO4 were identified to have significant effects on nisin biosynthesis by a Plackett–Burman design. These three significant factors were subsequently optimized using central composite design, and the optimal conditions were determined to be 12.067 g l−1 for yeast extract, 0.569 g l−1 for KH2PO4, and 0.572 g l−1 for MgSO4. The validity of the optimal conditions was verified by a separate experiment.

experimental designfermentationnisinoptimizationwhey

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Chuanbin Liu
    • 1
  • Yan Liu
    • 1
  • Wei Liao
    • 1
  • Zhiyou Wen
    • 1
  • Shulin Chen
    • 1
  1. 1.Department of Biological Systems EngineeringWashington State UniversityPullmanUSA