Plant Molecular Biology

, Volume 52, Issue 2, pp 371–386

Identification of a novel sugar transporter homologue strongly expressed in maturing stem vascular tissues of sugarcane by expressed sequence tag and microarray analysis

  • Rosanne E. Casu
  • Christopher P.L. Grof
  • Anne L. Rae
  • C. Lynne McIntyre
  • Christine M. Dimmock
  • John M. Manners
Article

DOI: 10.1023/A:1023957214644

Cite this article as:
Casu, R.E., Grof, C.P., Rae, A.L. et al. Plant Mol Biol (2003) 52: 371. doi:10.1023/A:1023957214644

Abstract

The ability of sugarcane to accumulate sucrose provides an experimental system for the study of gene expression determining carbohydrate partitioning and metabolism. A sequence survey of 7242 ESTs derived from the sucrose-accumulating, maturing stem revealed that transcripts for carbohydrate metabolism gene sequences (CMGs) are relatively rare in this tissue. However, within the CMG group, putative sugar transporter ESTs form one of the most abundant classes observed. A combination of EST analysis and microarray and northern hybridization revealed that one of the putative sugar transporter types, designated PST type 2a, was the most abundant and most strongly differentially expressed CMG in maturing stem tissue. PST type 2a is homologous to members of the major facilitator super-family of transporters, possessing 12 predicted transmembrane domains and a sugar transport conserved domain, interrupted by a large cytoplasmic loop. Its transcript was localized to phloem companion cells and associated parenchyma in maturing stem, suggesting a role in sugar translocation rather than storage. In addition, other categories of CMGs show evidence of coordinated expression, such as enzymes involved in sucrose synthesis and cleavage, and a majority of enzymes involved in glycolysis and the pentose phosphate pathway. This study demonstrates the utility of genomic approaches using large-scale EST acquisition and microarray hybridization techniques for studies of the developmental regulation of metabolic enzymes and potential transporters in sugarcane.

carbohydrate metabolism EST sequencing microarrays sugarcane sugar transport 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Rosanne E. Casu
    • 1
  • Christopher P.L. Grof
    • 1
  • Anne L. Rae
    • 1
  • C. Lynne McIntyre
    • 1
  • Christine M. Dimmock
    • 1
  • John M. Manners
    • 1
  1. 1.CSIRO Plant IndustryIndooroopillyAustralia

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