Biochemistry (Moscow)

, Volume 68, Issue 5, pp 529–533

β-1,3-Glucanase from Unfertilized Eggs of the Sea Urchin Strongylocentrotus intermedius. Comparison with β-1,3-Glucanases of Marine and Terrestrial Mollusks

  • V. V. Sova
  • N. I. Shirokova
  • M. I. Kusaykin
  • A. S. Scobun
  • L. A. Elyakova
  • T. N. Zvyagintseva
Article

DOI: 10.1023/A:1023951525250

Cite this article as:
Sova, V.V., Shirokova, N.I., Kusaykin, M.I. et al. Biochemistry (Moscow) (2003) 68: 529. doi:10.1023/A:1023951525250

Abstract

β-1,3-Glucanase (Lu) was isolated from unfertilized eggs of the sea urchin Strongylocentrotus intermedius. A comparative study of some properties of β-1,3-glucanase Lu and β-1,3-glucanases with different action types—endo-β-1,3-glucanase from crystalline style of the marine mollusk Spisula sachalinensis (LIV) and exo-β-1,3-glucanase from the terrestrial snail Eulota maakii (LII)—was performed. It was found that β-1,3-glucanase Lu hydrolyzes laminaran with a high yield of glucose in the reaction products. The enzyme hydrolyzes substrates with retention of the glycosidic bond configuration, is able to cleave modified substrates, and exhibits transglycosylating activity. All properties of β-1,3-glucanase from S. intermedius were more similar to those of the endo-β-1,3-glucanase from the marine mollusk (LIV) than exo-β-1,3-glucanase LII from the terrestrial snail. The differences in the effect of LIV and Lu on laminaran are probably related to the functions of β-1,3-glucanase Lu from sea urchin eggs (which, in contrast to LIV, is not a digestive enzyme).

β-1,3-glucanaseaction typesea urchin eggsStrongylocentrotus intermediusSpisula sachalinensisEulota maakii

Copyright information

© MAIK “Nauka/Interperiodica” 2003

Authors and Affiliations

  • V. V. Sova
    • 1
  • N. I. Shirokova
    • 1
  • M. I. Kusaykin
    • 1
  • A. S. Scobun
    • 2
  • L. A. Elyakova
    • 1
  • T. N. Zvyagintseva
    • 1
  1. 1.Far East Division, Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159Pacific Institute of Bioorganic ChemistryVladivostokRussia
  2. 2.Far East State Technical Fisheries University, ul. Lugovaya 52bVladivostokRussia