Plant Cell, Tissue and Organ Culture

, Volume 74, Issue 2, pp 123–134

Marker-free transgenic plants


DOI: 10.1023/A:1023934807184

Cite this article as:
Puchta, H. Plant Cell, Tissue and Organ Culture (2003) 74: 123. doi:10.1023/A:1023934807184


Selectable marker genes are widely used for the efficient transformation of crop plants. In most cases, selection is based on antibiotic or herbicide resistance. Due mainly to consumer concerns, a suite of strategies (site-specific recombination, homologous recombination, transposition and co-transformation) have been developed to eliminate the marker gene from the nuclear or chloroplast genome after selection. Current efforts concentrate on systems where marker genes are eliminated efficiently soon after transformation. Alternatively, transgenic plants are produced by the use of marker genes that do not rely on antibiotic or herbicide resistance but instead promote regeneration after transformation. Here, the merits and shortcomings of different approaches and possible directions for their future development are discussed.

homologous recombinationinductionnon-homologous end joiningscreenable markerssite-specific recombinationtransformationtransposition

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.Botany IIUniversität KarlsruheKarlsruheGermany