Combined Effect of Hydrogen Peroxide Induced Oxidative Stress and IL-1α on IL-8 Production in CaCo-2 Cells (a Human Colon Carcinoma Cell Line) and Normal Intestinal Epithelial Cells
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Oxidative stress, IL-1α, and IL-8 are known to contribute to mucosal inflammation of the gastrointestinal tract. We examined the IL-8 response after brief exposure to hydrogen peroxide induced oxidative stress in CaCo-2 cells (a human colon carcinoma cell line) and in human intestinal epithelial cells. In addition, we examined whether exposure to oxidative stress, followed by IL-1α, could modulate IL-8 production. A transient up-regulation of IL-8 mRNA expression was observed after hydrogen peroxide treatment. Hydrogen peroxide induced oxidative stress was also observed to promote IL-8 secretion. Exposure to hydrogen peroxide, followed by IL-1α, enhanced IL-8 production over that achieved with IL-1α alone. Thus, oxidative stress and IL-1α were observed to cooperatively enhance IL-8 production.
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- Combined Effect of Hydrogen Peroxide Induced Oxidative Stress and IL-1α on IL-8 Production in CaCo-2 Cells (a Human Colon Carcinoma Cell Line) and Normal Intestinal Epithelial Cells
Volume 27, Issue 3 , pp 123-128
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- oxidative stress
- hydrogen peroxide
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- Author Affiliations
- 1. Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Seta-tsukinowa, Ohtsu, Shga, Japan
- 2. Japan Clinical Laboratories, Inc., Kumiyama, Kuze, Kyoto, Japan
- 3. Department of Gastroenterology, Shiga University of Medical science, Seta-tsukinowa, Ohtsu, Shga, Japan