World Journal of Microbiology and Biotechnology

, Volume 19, Issue 3, pp 243–246

Activated charcoal: a versatile decolorization agent for the recovery and purification of alkaline protease

  • C. Ganesh Pradeep Kumar

DOI: 10.1023/A:1023644615956

Cite this article as:
Kumar, C.G.P. World Journal of Microbiology and Biotechnology (2003) 19: 243. doi:10.1023/A:1023644615956


The use of activated charcoal for enzyme recovery and purification was investigated and the optimum activated charcoal concentration and the minimum contact time needed for efficient decolorization of an alkaline protease preparation in terms of surface adsorption and retention of enzyme activity were found to be 7.5 g l−1 and 30 min, respectively. Elevated temperatures had a greater influence on the rate of decolorization which was faster when the protease was refluxed at 60 °C for 10–15 min. These data suggest that the efficient adsorption characteristics of activated charcoal can be exploited for cost-effective downstream processing of alkaline proteases and possibly other enzymes.

Activated charcoalalkaline proteasedecolorizationdownstream processingpurification

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • C. Ganesh Pradeep Kumar
    • 1
    • 2
    • 1
  1. 1.Department of BiochemistryCollege of Medicine, Inha UniversityaKorea
  2. 2.Department of BiochemistryBoseCalcuttaIndia; Tel.: