World Journal of Microbiology and Biotechnology

, Volume 19, Issue 3, pp 263–268

Production and characterization of thermostable cellulases from Streptomyces transformant T3-1

  • Hung-Der Jang
  • Kuo-Shu Chen
Article

DOI: 10.1023/A:1023641806194

Cite this article as:
Jang, HD. & Chen, KS. World Journal of Microbiology and Biotechnology (2003) 19: 263. doi:10.1023/A:1023641806194

Abstract

A total of 26 thermophilic isolates, selected from a compost of agricultural waste, which was mostly composed of vegetable, corncob and rice straw, were cultivated at 50 °C for further studies of thermostable cellulase production. The thermostable cellulase gene from the chromosomal DNA of actinomycetes isolate no. 10 was shotgun-cloned and transformed into Streptomyces sp. IAF 10-164. A transformant, T3-1, was found to be a good strain for the production of thermostable cellulases. Cultivation of T3-1 in modified Mandels–Reese broth containing 1% carboxymethylcellulose (CMC)-sodium salt and the optimal condition for microbial growth were studied. Batch cultivation in a flask revealed that CMCase and Avicelase production reached the maximum between the third to fifth day, whereas maximum β-glucosidase production occurred on the ninth day. Microbial biomass increased from the first day to the fifth day and then decreased. The crude enzyme had the highest activity at 50 °C and at pH 6.5. The enzyme was shown to be a thermostable cellulase whose activities were stable at 50 °C for more than 7 days.

Compost gene cloning Streptomyces thermostable cellulase transformants 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Hung-Der Jang
    • 1
  • Kuo-Shu Chen
    • 2
  1. 1.Department of Food ScienceYuanpei University of Science and TechnologyHsinchuTaiwan; Tel.:
  2. 2.Department of Medical TechnologyYuanpei University of Science and TechnologyHsinchuTaiwan