, Volume 39, Issue 3, pp 257-264

Isolation and Characterization of Humin-Like Substances Produced by Wood-Degrading White Rot Fungi

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Three samples of high-molecular-weight humin-like substances were obtained by solid-phase cultivation of Coriolus hirsutus and/or Cerrena maxima on oat straw. The yield of humin-like substances amounted to 1.38–2.26% of the weight of the plant substrate consumed. These substances, produced both by individual and mixed cultures of the basidiomycetes, were shown to be similar in their structure and physicochemical properties. According to the data of IR and 13C-NMR spectroscopy, the substances contained aromatic fragments and were close to soil humic acids. Studies of the dynamics of laccase production suggested that the humin-like substances were produced via direct degradation of lignin macromolecules with direct involvement of extracellular laccase.