Antonie van Leeuwenhoek

, Volume 83, Issue 2, pp 155–166

Differentiation of six sibling species in the Saccharomyces sensu stricto complex by multilocus enzyme electrophoresis and UP-PCR analysis

  • E.S. Naumova
  • S.A. Bulat
  • N.V. Mironenko
  • G.I. Naumov
Article

DOI: 10.1023/A:1023328320228

Cite this article as:
Naumova, E., Bulat, S., Mironenko, N. et al. Antonie Van Leeuwenhoek (2003) 83: 155. doi:10.1023/A:1023328320228

Abstract

UP-PCR analysis and multilocus enzyme electrophoresis were used to characterize 37 strains of the sibling species Saccharomyces cerevisiae, S. bayanus, S. cariocanus, S. kudriavzevii, S. mikatae and S. paradoxus. The results demonstrate that both molecular approaches are useful for discriminating between these phenotypically indistinguishable Saccharomyces species. The data obtained are in excellent agreement with previously reported genetic analyses, sequencing of the 18S rRNA and ITS regions, and DNA-DNA reassociation data.

Biological speciesMLEESaccharomyces sensu strictoUP-PCR

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • E.S. Naumova
    • 1
  • S.A. Bulat
    • 2
  • N.V. Mironenko
    • 3
  • G.I. Naumov
    • 1
  1. 1.State Institute for Genetics and Selection of Industrial MicroorganismsMoscowRussia
  2. 2.Petersburg Nuclear Physics InstituteGatchinaRussia
  3. 3.All-Russian Plant Protection InstituteSt. Petersburg-PushkinRussia