Behaviour of antioxidant defense system in the adaptive response to salt stress in Helianthus annuus L. cells
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A relationship between the antioxidant defense system and salt tolerance in two types of sunflower calli differing in salt sensitivity was studied. No reduction in growth occurred in the NaCl-salt-adapted cell line (T) when grown on 175 mM NaCl but growth of the salt-stressed cell line (S) was reduced by 83%. Lipid peroxidation and protein oxidation increased during acute stress of salt stressed cells at 14 and 28 d of the experiment, while salt-adapted calli (T) remained similar to non-shocked (C) values. The antioxidant defense system of callus adapted to growth under NaCl responded differently to 175 mM of salt compared with the corresponding controls under shock treatment. Salt-adapted and salt-stressed calli showed a similar pattern in GSH content at day 14 but at day 28 in S calli, GSH content was increased 100% over the non-shocked calli, while T calli returned to the initial values. In the salt-stressed calli, a general decrease in all the antioxidant enzymes studied (except for glutathione reductase and dehydroascorbate reductase activities) was observed at day 28. Except for catalase, the antioxidant enzymes were elevated constitutively in adapted calli as compared to stressed cells, when both were grown in the absence of NaCl (time 0), and remained unaltered until 28 d after the beginning of the experiment. These results suggest the involvement of an enzymatic antioxidant defense system in the adaptive response to salt stress in Helianthus annuus L. cells.
- Ahmad S. 1995. Oxidative Stress and Antioxidant Defenses in Biology. Chapman & Hall., New York.
- Anderson M.E. 1985. Determination of glutathione and glutathione disulfide in biological samples. Meth. Enzymol. 113: 548–554.
- Becana M., Aparico-Tejo P., Irigoyen J.J. and Sanchez-Diaz M. 1986. Some enzymes of hydrogen peroxide metabolism in leaves and root nodules of Medicago sativa. Plant Physiol. 82: 1169–1171.
- Bellaire B.A., Carmody J., Braud J., Gosset D.R., Banks S.W., Cran Lucas M. et al. 2000. Involvement of abscisic acid-dependent and independent pathways in the upregulation of antioxidant enzyme activity during NaCl stress in cotton callus tissue. Free Rad. Res. 33: 531–545.
- Benavides M.P., Marconi P.L., Gallego S.M., Comba M.E. and Tomaro M.L. 2000. Relationship between antioxidant defence systems and salt tolerance in Solanum tuberosum. Aus. J. Plant Physiol. 27: 273–278.
- Chance B., Sies H. and Boveris A. 1979. Hydroperoxide metabolism in mammalian organs. Physiol. Rev. 59: 527–605.
- Comba M.E., Benavides M.P. and Tomaro M.L. 1998. Effect of salt stress on antioxidant defence system in soybean root nodules. Aus. J. Plant Physiol. 25: 665–671.
- Gallego S.M., Benavides M.P. and Tomaro M.L. 1996. Effect of heavy metal ion excess on sunflower leaves: evidence for involvement of oxidative stress. Plant Sci. 121: 151–159.
- Giannopolitis C.N. and Ries S.K. 1977. Superoxide Dismutase, I. Occurrence in higher plants. Plant Physiol. 59: 309–314.
- Gosset D.R., Baris S.W., Millhollon E.P. and Cran Lucas M. 1996. Antioxidant response to NaCl stress in a control and an NaCltolerant cotton cell line grown in the presence of paraquat, buthionine sulfoximine, and exogenous glutathione. Plant Physiol. 112: 803–809.
- Gueta-Dahan Y., Yaniv Z., Zilinskas B.A. and Ben-Hayyim G. 1997. Salt and oxidative stress: similar and specific responses and their relation to salt tolerance in Citrus. Planta 203: 460–469.
- Heath R.L. and Packer L. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125: 189–198.
- Hernadez J.A., Jimenez A., Mullineaux P. and Sevilla F. 2000. Tolerance of pea (Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defences. Plant Cell. Environm. 23: 853–862.
- Hernandez J.A., Corpas F.J., Gomez M., del Rio L.A. and Sevilla F. 1993. Salt-induced oxidative stress mediated by activated oxygen species en pea leaf mitochondria. Physiol. Plantarum 89: 103–110.
- Hernandez J.A., Olmos E., Corpas F.J., Sevilla F. and del Rio L.A. 1995. Salt-induced oxidative stress in chloroplast of pea plants. Plant Sci. 105: 151–167.
- Levine R.L., Garland D., Oliver C.N., Amici A., Climent I., Lenz A.G. et al. 1990. Determination of carbonyl content in oxidatively modified protein. Meth. Enzymol. 186: 464–478.
- Lopez F., Vansuyt G., Casse-Delbart F. and Fourcroy P. 1996. Ascorbate peroxidase activity, not the mRNA level, is enhanced in salt-stress Raphanus sativus plants. Physiol. Plantarum 97: 13–20.
- Munns R. 1993. Physiological processes limiting plant growth in saline soils: some dogmas and hypothesis. Plant Cell Environ. 16: 15–24.
- Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plantarum 15: 473–497.
- Nakano Y. and Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplast. Plant Cell Physiol. 22: 867–880.
- Noctor G. and Foyer C.H. 1998. Ascorbate and glutathione: Keeping active oxygen under control. Ann. Rev. Plant Physiol. Plant Mol. Biol. 49: 249–279.
- Scandalio L.M., Dalurzo H.C., Gómez M., Romero-Puertas M.C. and del Río L.A. 2001. Cadmium-induced changes in the growth and oxidative metabolism of pea plants. J. Exp. Bot. 52: 2115–2126.
- Schaedle M. and Bassham J.A. 1977. Chloroplast glutathione reductase. Plant Physiol. 59: 1011–1012.
- Schupp R. and Rennenberg H. 1988. Diurnal changes in the glutathione content of spruce needles (Picea abies L.). Plant Sci. 57: 113–117.
- Screenivasulu N., Grimm B., Wobus U. and Weschke W. 2000. Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedling of foxtail millet (Setaria italica). Physiol. Plantarum 109: 435–442.
- Singha S. and Choudhuri M.A. 1990. Effect of salinity (NaCl) stress on H2O2 metabolism in Vigna and Oryza seedlings. Biochem. Physiol. Pflanz 186: 69–74.
- Smirnoff N. 1998. Plant resistance to environmental stress. Cur. Opin. Biotech. 9: 214–219.
- Tsugane K., Kobayashi K., Niwa Y., Ohba Y., Wada K. and Kobayashi H. 1999. A recessive Arabidopsis mutant that grows photoautotrophically under salt stress shows enhance active oxygen detoxification. Plant Cell 7: 1195–1206.
- Behaviour of antioxidant defense system in the adaptive response to salt stress in Helianthus annuus L. cells
Plant Growth Regulation
Volume 40, Issue 1 , pp 81-88
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- Kluwer Academic Publishers
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- Antioxidant defenses
- Helianthus annuus L.
- Oxidative stress
- Salt stress
- Salt tolerance
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