Mycopathologia

, Volume 156, Issue 2, pp 133–138

Isolation and toxigenicity of Aspergillus fumigatus from moldy silage

  • Valentina Melo dos Santos
  • Joe W. Dorner
  • Fátima Carreira
Article

DOI: 10.1023/A:1022996911563

Cite this article as:
Melo dos Santos, V., Dorner, J.W. & Carreira, F. Mycopathologia (2003) 156: 133. doi:10.1023/A:1022996911563

Abstract

Thirty-nine silage samples were collected from various siloson Terceira Island in the Azores. Samples were examined for the presence of total fungi, and isolates of Aspergillus fumigatus were analyzed for their ability to produce fumitremorgens B and C, fumigaclavines B and C, and gliotoxin. Thirty-four silage samples (87%) were contaminated with fungi, and A. fumigatus was isolated from 27 samples (69%). Samples that were taken from the surface of silos had significantly higher populations of both total fungi and A. fumigatus than did samples taken from the middle of silos. Analysis of 27 A. fumigatus isolates (one representing each positive sample) showed that 59.3% produced fumitremorgen B; 33.3% produced fumitremorgen C; 29.6% produced fumigaclavine B; 7.4% produced fumigaclavine C; and 11.1% produced gliotoxin. Fifty-two percent of the isolates produced multiple toxins, and 25.9% did not produce any of these toxins. Gliotoxin and fumigaclavine C were always produced in combination with other toxins. Because of the demonstrated potential of these A. fumigatus isolates to producemycotoxins, it is important to properly construct and manage silos to prevent their contamination with A. fumigatus.

Aspergillus fumigatusfumigaclavinefumitremorgengliotoxinmycotoxinssilage

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Valentina Melo dos Santos
    • 1
  • Joe W. Dorner
    • 2
  • Fátima Carreira
    • 1
  1. 1.Sector de Micologia, Laboratório Regional de VeterináriaAngra do Heroísmo, AçoresPortugal
  2. 2.National Peanut Research LaboratoryDawsonUSA