, Volume 156, Issue 2, pp 81–85

Mycoflora of tuber surface of white yam (Dioscorea rotundata poir) and postharvest control of pathogens with Bacillus subtilis

  • Ralph N. Okigbo

DOI: 10.1023/A:1022976323102

Cite this article as:
Okigbo, R.N. Mycopathologia (2003) 156: 81. doi:10.1023/A:1022976323102


Bacillus subtilis (Enrenberg) Cohn was investigated for its antagonistic properties against surface mycoflora of yam (Dioscorea rotundata Poir) tubers in storage. Yam tubers inoculated with a spore suspension of B. subtilis in potato dextrose broth using a knapsack sprayer showed a drastic reduction in the range and number of mycoflora, including pathogens of the tuber surface in contrast to the control tubers, during the five-month storage period in a traditional yam barn. However, B. subtilis maintained a high frequency of occurrence during the same period. Botryodiploidia theobromae Pat, Fusarium moniliforme Wollen and Reink., Penicillium sclerotigenum Yamamoto, and Rhizoctonia sp. were displaced completely on the treated tubers. The antagonism of B. subtilis was so effective that the normal tuber surface mycoflora was greatly reduced throughout the storage period of five months by a simple initial application of the antagonist.

Bacillus subtilisBiological controlmycoflorarotyam

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Ralph N. Okigbo
    • 1
  1. 1.Department of Biological SciencesMichael Okpara University of AgricultureUmuahia, Abia StateNigeria