Plant and Soil

, Volume 251, Issue 1, pp 47–54

Colonization behaviour of Pseudomonas fluorescens and Sinorhizobium meliloti in the alfalfa (Medicago sativa) rhizosphere

  • Marta Villacieros
  • Barry Power
  • María Sánchez-Contreras
  • Javier Lloret
  • Roke I. Oruezabal
  • Marta Martín
  • Francisca Fernández-Piñas
  • Ildefonso Bonilla
  • Clare Whelan
  • David N. Dowling
  • Rafael Rivilla
Article

DOI: 10.1023/A:1022943708794

Cite this article as:
Villacieros, M., Power, B., Sánchez-Contreras, M. et al. Plant and Soil (2003) 251: 47. doi:10.1023/A:1022943708794

Abstract

The colonization ability of Pseudomonas fluorescens F113rif in alfalfa rhizosphere and its interactions with the alfalfa microsymbiont Sinorhizobium meliloti EFB1 has been analyzed. Both strains efficiently colonize the alfalfa rhizosphere in gnotobiotic systems and soil microcosms. Colonization dynamics of F113rif on alfalfa were similar to other plant systems previously studied but it is displaced by S. meliloti EFB1, lowering its population by one order of magnitude in co-inoculation experiments. GFP tagged strains used to study the colonization patterns by both strains indicated that P. fluorescens F113rif did not colonize root hairs while S. meliloti EFB1 extensively colonized this niche. Inoculation of F113rif had a deleterious effect on plants grown in gnotobiotic systems, possibly because of the production of HCN and the high populations reached in these systems. This effect was reversed by co-inoculation. Pseudomonas fluorescens F113 derivatives with biocontrol and bioremediation abilities have been developed in recent years. The results obtained support the possibility of using this bacterium in conjunction with alfalfa for biocontrol or rhizoremediation technologies.

colonizationgreen fluorescent proteinPseudomonas fluorescensrhizosphereSinorhizobium meliloti

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Marta Villacieros
    • 1
    • 2
  • Barry Power
    • 2
  • María Sánchez-Contreras
    • 1
  • Javier Lloret
    • 1
  • Roke I. Oruezabal
    • 1
  • Marta Martín
    • 1
  • Francisca Fernández-Piñas
    • 1
  • Ildefonso Bonilla
    • 1
  • Clare Whelan
    • 2
  • David N. Dowling
    • 2
  • Rafael Rivilla
    • 1
  1. 1.Departamento de BiologíaUniversidad Autónoma de MadridMadridSpain
  2. 2.Department of Applied Biology and ChemistryInstitute of Technology, CarlowCarlowIreland