Plant Cell, Tissue and Organ Culture

, Volume 73, Issue 2, pp 159–166

Production of seedlings from ovules excised at the zygote stage in Lilium spp.


DOI: 10.1023/A:1022818413617

Cite this article as:
Ikeda, N., Niimi, Y. & Han, DS. Plant Cell, Tissue and Organ Culture (2003) 73: 159. doi:10.1023/A:1022818413617


The present study was undertaken to establish a culture system for ovules excised at the zygote stage in Lilium spp. Ovules of Lilium × `Connecticut King' and L. × `Enchantment' were excised together with placental tissue 3, 5, and 10 days after pollination (DAP) and cultured on B5 medium and half-strength B5 medium containing sucrose at different concentrations. In vitro embryo development in ovules cultured at 3 DAP was influenced by the basal media and the sucrose concentration. The half-strength B5 medium with 9% sucrose was the best condition, but only a few ovules isolated from placental tissue developed into seedlings. Application of embryo culture, in which embryos were excised from ovules after 14 weeks of ovule-with-plancetal-tissue culture, greatly improved the production of seedlings. The present study indicates that a two-step culture procedure, ovule-with-placental-tissue culture and embryo culture, make it possible to produce seedlings from ovules just after fertilization.

in vitro embryo developmentlilysucrose concentration

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.Faculty of AgricultureNiigata UniversityNiigataJapan