Journal of Bioenergetics and Biomembranes

, Volume 34, Issue 6, pp 487–498

The Energy-Conserving and Energy-Dissipating Processes in Mitochondria Isolated from Wild Type and Nonripening Tomato Fruits During Development on the Plant

  • Andréa Miyasaka Almeida
  • Rachel Navet
  • Wieslawa Jarmuszkiewicz
  • Anibal E. Vercesi
  • Claudined M. Sluse-Goffart
  • Francis E. Sluse
Article

DOI: 10.1023/A:1022574327117

Cite this article as:
Almeida, A.M., Navet, R., Jarmuszkiewicz, W. et al. J Bioenerg Biomembr (2002) 34: 487. doi:10.1023/A:1022574327117

Abstract

Bioenergetics of tomato (Lycopersicon esculentum) development on the plant was followed from the early growing stage to senescence in wild type (climacteric) and nonripening mutant (nor, nonclimacteric) fruits. Fruit development was expressed in terms of evolution of chlorophyll a content allowing the assessment of a continuous time-course in both cultivars. Measured parameters: the cytochrome pathway-dependent respiration, i.e., the ATP synthesis-sustained respiration (energy-conserving), the uncoupling protein (UCP) activity-sustained respiration (energy-dissipating), the alternative oxidase(AOX)-mediated respiration (energy-dissipating), as well as the protein expression of UCP and AOX, and free fatty acid content exhibited different evolution patterns in the wild type and nor mutant that can be attributed to their climacteric/nonclimacteric properties, respectively. In the wild type, the climacteric respiratory burst observed in vitro depended totally on an increse in the cytochrome pathway activity sustained by ATP synthesis, while the second respiratory rise during the ripening stage was linked to a strong increase in AOX activity accompanied by an overexpression of AOX protein. In wild type mitochondria, the 10-μM linoleic acid-stimulated UCP-activity-dependent respiration remained constant during the whole fruit development except in senescence where general respiratory decay was observed.

Alternative oxidase uncoupling protein mitochondria respiration tomato fruit ripening free fatty acids 

Copyright information

© Plenum Publishing Corporation 2002

Authors and Affiliations

  • Andréa Miyasaka Almeida
    • 1
  • Rachel Navet
    • 1
  • Wieslawa Jarmuszkiewicz
    • 2
  • Anibal E. Vercesi
    • 3
  • Claudined M. Sluse-Goffart
    • 1
  • Francis E. Sluse
    • 1
  1. 1.Laboratory of Bioenergetics, Department of Life Sciences, Institute of Chemistry B6University of Liége, Sart TilmanLiégeBelgium
  2. 2.Department of Bioenergetics, Institute of Molecular Biology and BiotechnologyAdam Mickiewicz UniversityPoznanPoland
  3. 3.Departamento de Patologia ClínicaUniversidade Estadual de CampinasCampinas, SPBrazil