Successful Use of a Laser for Human Embryo Biopsy in Preimplantation Genetic Diagnosis: Report of Two Cases

  • M. Boada
  • M. Carrera
  • C. De La Iglesia
  • M. Sandalinas
  • P. N. Barri
  • A. Veiga
Article

DOI: 10.1023/A:1022548612107

Cite this article as:
Boada, M., Carrera, M., De La Iglesia, C. et al. J Assist Reprod Genet (1998) 15: 302. doi:10.1023/A:1022548612107

Abstract

Purpose:The use of Tyrode's acid to drill the zona pellucida for embryo biopsy is the most widely used methodology in preimplantation genetic diagnosis. Instead of this, we propose the use of a 1.48-μm diode noncontact laser, which is quicker, simpler, and safer.

Methods:The laser beam was tangentially guided to the zona pellucida of the embryo. Depending on zona pellucida measurement, two to four consecutive shots of 8–22 msec were necessary to drill the zona pellucida of the 13 embryos biopsied for two patients (hemophilia carriers).

Results:Female embryos were replaced into the uterus of the patients (1.5 embryos/replacement). One single pregnancy was established (33.3% implantation rate). Coculture of untransferable embryos showed a blastocyst rate of 66.7% (4/6) for male embryos and 25% (1/4) for abnormal ones.

Conclusions:These results demonstrate the safety and usefulness of laser methodology in preimplantation genetic diagnosis.

diode laser beam embryo biopsy microdrilling preimplantation diagnosis zona pellucida 

Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • M. Boada
    • 1
  • M. Carrera
    • 2
  • C. De La Iglesia
    • 2
  • M. Sandalinas
    • 1
  • P. N. Barri
    • 1
  • A. Veiga
    • 1
  1. 1.Servei de Medicina de la Reproducció, Departament d'Obstetrícia i GinecologiaInstitut Universitari DexeusBarcelonaSpain
  2. 2.Centro de Patología CelularInstitut Universitari DexeusBarcelonaSpain