Journal of Assisted Reproduction and Genetics

, Volume 15, Issue 8, pp 513–520

Immunohistochemical Analysis of Insulin-like Growth Factor–Binding Proteins -1, -2, and -3 in Implantation Sites of the Mouse

  • Mark A. Damario
  • Hung-Ching Liu
  • Carol Ann Mele
  • Marcelo G. Horenstein
  • Zev Rosenwaks
Article

DOI: 10.1023/A:1022538621928

Cite this article as:
Damario, M.A., Liu, HC., Mele, C.A. et al. J Assist Reprod Genet (1998) 15: 513. doi:10.1023/A:1022538621928

Abstract

Purpose:Our purpose was to analyze potential interactions between the embryo and the maternal endometrial interface in vivo by analyzing immunolocalization of insulin-like growth factor–binding proteins (IGFBPs) -1, -2, and -3 in implantation sites of the mouse.

Methods:Six-week-old B6D2F1female mice underwent superovulation followed by mating and sacrifice at timed intervals. Formalin-fixed paraffin-embedded tissue was used for avidin–biotin immunocytochemical localization of IGFBPs utilizing standard methodology.

Results:Immunostaining at 1.5 days post coitum revealed light staining in the epithelial glandular cells and faint staining in decidual stroma for both IGFBP-1 and IGFBP-2. At 7.5–10.5 days post coitum, there was moderate–dense immunostaining in the decidualized stromal cells at the implantation site for all three IGFBPs, whereas light immunostaining was seen in nonimplantation site decidua.

Conclusions:Compartmentalization of immunostaining for IGFBP-1, -2, and -3 within decidualized stroma suggests that these proteins may be regulated by trophoblastic and/or embryonic signals.

deciduaimmunohistochemistryimplantationinsulin-like growth factor–binding proteins

Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Mark A. Damario
    • 1
  • Hung-Ching Liu
    • 1
  • Carol Ann Mele
    • 1
  • Marcelo G. Horenstein
    • 3
  • Zev Rosenwaks
    • 1
  1. 1.Center for Reproductive Medicine and Infertility, Department of Obstetrics and GynecologyCornell University Medical CollegeNew York
  2. 2.Mayo ClinicRochester
  3. 3.Department of PathologyCornell University Medical CollegeNew York
  4. 4.Department of PathologyDuke University Medical CenterDurham