Journal of Assisted Reproduction and Genetics

, Volume 15, Issue 8, pp 513–520

Immunohistochemical Analysis of Insulin-like Growth Factor–Binding Proteins -1, -2, and -3 in Implantation Sites of the Mouse

Authors

  • Mark A. Damario
    • Center for Reproductive Medicine and Infertility, Department of Obstetrics and GynecologyCornell University Medical College
  • Hung-Ching Liu
    • Center for Reproductive Medicine and Infertility, Department of Obstetrics and GynecologyCornell University Medical College
  • Carol Ann Mele
    • Center for Reproductive Medicine and Infertility, Department of Obstetrics and GynecologyCornell University Medical College
  • Marcelo G. Horenstein
    • Department of PathologyCornell University Medical College
  • Zev Rosenwaks
    • Center for Reproductive Medicine and Infertility, Department of Obstetrics and GynecologyCornell University Medical College
Article

DOI: 10.1023/A:1022538621928

Cite this article as:
Damario, M.A., Liu, H., Mele, C.A. et al. J Assist Reprod Genet (1998) 15: 513. doi:10.1023/A:1022538621928

Abstract

Purpose:Our purpose was to analyze potential interactions between the embryo and the maternal endometrial interface in vivo by analyzing immunolocalization of insulin-like growth factor–binding proteins (IGFBPs) -1, -2, and -3 in implantation sites of the mouse.

Methods:Six-week-old B6D2F1female mice underwent superovulation followed by mating and sacrifice at timed intervals. Formalin-fixed paraffin-embedded tissue was used for avidin–biotin immunocytochemical localization of IGFBPs utilizing standard methodology.

Results:Immunostaining at 1.5 days post coitum revealed light staining in the epithelial glandular cells and faint staining in decidual stroma for both IGFBP-1 and IGFBP-2. At 7.5–10.5 days post coitum, there was moderate–dense immunostaining in the decidualized stromal cells at the implantation site for all three IGFBPs, whereas light immunostaining was seen in nonimplantation site decidua.

Conclusions:Compartmentalization of immunostaining for IGFBP-1, -2, and -3 within decidualized stroma suggests that these proteins may be regulated by trophoblastic and/or embryonic signals.

deciduaimmunohistochemistryimplantationinsulin-like growth factor–binding proteins

Copyright information

© Plenum Publishing Corporation 1998