Journal of Protein Chemistry

, Volume 17, Issue 2, pp 143–148

Isolation and Characterization of Full and Truncated Forms of Human Breast Carcinoma Protein BA46 from Human Milk Fat Globule Membranes

  • Maria Gabriella Giuffrida
  • Maria Cavaletto
  • Carlo Giunta
  • Amedeo Conti
  • Jasminka Godovac-Zimmermann
Article

DOI: 10.1023/A:1022531500370

Cite this article as:
Giuffrida, M.G., Cavaletto, M., Giunta, C. et al. J Protein Chem (1998) 17: 143. doi:10.1023/A:1022531500370

Abstract

We have isolated and characterized two proteins of 50 and 30 kDa from human milk fat globule membranes of healthy donors. N-terminal and internal sequencing revealed that the 50-kDa protein is the full-length human breast carcinoma protein BA46 that is highly expressed in human breast tumors. The 30-kDa protein is a truncated form of protein BA46 which consists of the C-terminal factor V/VIII-like domain of BA46 and which appears to anchor BA46 to the milk fat globule membrane. Defective release of the epidermal growth factor domain containing a surface RGD motif may be related to involvement of BA46 in breast cancer

Human breast carcinomahuman milk fat globule membrane proteinsepidermal growth factorfactor V/VIII

Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Maria Gabriella Giuffrida
    • 1
    • 2
  • Maria Cavaletto
    • 3
  • Carlo Giunta
    • 3
  • Amedeo Conti
    • 2
  • Jasminka Godovac-Zimmermann
    • 1
  1. 1.Institut für Molekulare BiotechnologieJenaGermany
  2. 2.Centre Studio Alimentazione AnimaliTorinoItaly
  3. 3.Dipartimento di Biologia AnimaleUniversità di TorinoItaly