Establishment of the mimetic aging effect in mice caused by D-galactose
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- Ho, SC., Liu, JH. & Wu, RY. Biogerontology (2003) 4: 15. doi:10.1023/A:1022417102206
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We successfully established an oxidant damageof mimetic aging model using mice induced byD-galactose, and the mimetic aging model isrelative to free radical and the accumulationof waste substances in metabolism. The animalswere divided into 3 groups: (1)phosphate-buffered saline (PBS); (2) 1%D-galactose; (3) 5% D-galactose bysubcutaneous injection every day. After 45days, mice treated with D-galactose showed asignificant increase in the malondialdehyde(MDA), total antioxidant status (TAS) and adecrease in superoxide dismutase (SOD) in the blood comparedwith the PBS group. In the brain, theD-galactose treated mice exhibited a higherlevel MDA and a lower level SOD activity.In the liver, only the 5% D-galactose groupindicated a significant increase in MDA. By reference to theoxidative biomarkers in blood, brain and liver,we have confirmed the reliability of themimetic aging model.