Inflammation

, Volume 22, Issue 3, pp 307–314

Arachidonic Acid (AA) and Tetradecanoylphorbol Acetate (TPA) Exert Systemic Effects When Applied Topically in the Mouse

  • V. Puigneró
  • A. Turull
  • J. Queralt
Article

DOI: 10.1023/A:1022352200273

Cite this article as:
Puigneró, V., Turull, A. & Queralt, J. Inflammation (1998) 22: 307. doi:10.1023/A:1022352200273

Abstract

We studied the systemic actions of topically applied arachidonic acid (AA) and tetradecanoylphorbol acetate (TPA) in the mouse. AA or TPA-induced ear edema, increases in vascular permeability, eicosanoid levels, neutrophil and mononuclear influx were determined in both phlogogen-treated and contralateral vehicle-treated ear of each mouse and were compared with vehicle-treated ears from control mice. Edema and vascular permeability increases appeared only in AA- or TPA-applied ears. Moreover, in contralateral ears from AA-treated mice an increase in 6-keto-PGF and LTB4 was found. Only LTB4 increased in the contralateral ear after TPA. Contralateral ears from AA- or TPA-treated mice also showed a significant increase in MPO levels. The increased levels of 6-keto-PGF but not those of LTB4 in contralateral ears were reduced by indomethacin applied simultaneously with the phlogogen. AA also increased plasma and serum levels of LTB4, but not those of 6-keto-PGF. In contrast, TPA increased plasma and serum levels of 6-keto-PGF and LTB4. The results show that both AA and TPA applied topically exert systemic effects.

Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • V. Puigneró
    • 1
  • A. Turull
    • 1
  • J. Queralt
    • 1
  1. 1.Unitat de Fisiologia.Universitat de Barcelona.Barcelona.Spain