By-product formation by a novel glycerol-producing yeast, Candida glycerinogenes, with different O2 supplies
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Candida glycerinogenes is an aerobe which does not depend on sulphite for production of glycerol. With a sufficient O2 supply, up to 130 g glycerol l−1 was produced with 2.6 g acetic acid l−1 as by-product. However, with an insufficient O2 supply – with higher volumes of medium or at higher corn steep liquid concentrations – the glycerol concentration was lower because the by-products, ethanol, pyruvate and lactic acid, were produced in greater amounts, up to 45 g l−1, 4.3 g l−1, 1.6 g l−1, respectively, whereas, less acetic acid (0.6 g l−1) was produced. In addition, ethanol decreased to 0.4 g l−1 and the glycerol yield improved from 34 to 50% (w/w) by adding 50 g sulphite l−1, nevertheless, acetic acid increased to 7.8 g l−1.
- Agarwal GP (1990) Glycerol. In: A. Fiechter, ed. Advance in Biotechnological Engineering/Biotechnology: Microbial Bioproducts, Vol. 41. Berlin, Heidelberg: Springer-Verlag, pp. 95–128.
- Amata IA, Germain P (1990) The effect of pitching yeast aeration on the production of acetic acid during fermentations with brewers' yeast: an enzymatic approach. J. Inst. Brew. 96: 131–134.
- Fan D-d, Yu J-T, Wei D-Z (1994) Determination of O2 permeability and volumetric O2 transfer rate of shaken flasks. Chin. J. Biotechnol. 10: 114–117.
- Hajny GJ (1960) Factors affecting glycerol production by a newly isolated osmophilic yeast. Appl. Microbiol. 8: 5–11.
- Jin H-R, Zhuge J (2000) Kinds and amount variation of organic acids during microbial glycerol production with Candia glycerinogenes. J. Wuxi Univ. Light Ind. 19: 205–208.
- Jin H-R, Zhuge J (2001) The determination of the key component showing acidific in commercial glycerol by fermentation. J. Wuxi Univ Light Ind. 20: 125–128.
- Liu Y-Q, Liu D-H, Su Q, Xie D-M (2002) Glycerol production by Candida krusei employing NaCl as an osmoregulator in batch and continuous fermentations. Biotechnol. Lett. 24: 1137–1140.
- Michnick S, Roustan IL, Dequin S (1997) Modulation of glycerol and ethanol yields during alcoholic fermentation in Saccharomyces cerevisiae strains over expressed or disrupted for GPD1 encoding glycerol-3-phosphate dehydrogenase. Yeast 13: 783–797.
- Petrovska B, Winkelhausen E, Kuzmanova S (1999) Glycerol production by yeast under osmotic and sulfite stress. Can. J. Microbiol. 45: 695–699.
- Sahoo DK (1990) The effect of oxygen on the production of polyols by Candida magnoliae. Indian Chem. Eng. 32: 75–81.
- Vijaikishore P, Karanth NG (1986) Factors affecting glycerol production by Pichia farinosa under alkaline condition. Appl. Biochem. Biotechnol. 13: 189–205.
- Wang Z-X, Zhuge J, Prior BA (2001) Glycerol production by microbial fermentation: a review. Biotechnol. Adv. 19: 210–223.
- Zhuge J, Jin H-R, Gu H-L (2001) Glycerol production by a novel osmotolerant yeast Candida glycerinogenes. Appl. Microbiol. Biotechnol. 55: 686–692.
- By-product formation by a novel glycerol-producing yeast, Candida glycerinogenes, with different O2 supplies
Volume 25, Issue 4 , pp 311-314
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- osmotolerant yeast
- O2 supply
- Industry Sectors