Plant Molecular Biology

, Volume 51, Issue 4, pp 577–587

Identification of a copper transporter family in Arabidopsis thaliana

  • Vicente Sancenón
  • Sergi Puig
  • Helena Mira
  • Dennis J. Thiele
  • Lola Peñarrubia
Article

DOI: 10.1023/A:1022345507112

Cite this article as:
Sancenón, V., Puig, S., Mira, H. et al. Plant Mol Biol (2003) 51: 577. doi:10.1023/A:1022345507112

Abstract

Despite copper ions being crucial in proteins participating in plant processes such as electron transport, free-radical elimination and hormone perception and signaling, very little is known about copper inward transport across plant membranes. In this work, a five-member family (COPT1–5) of putative Arabidopsis copper transporters is described. We ascertain the ability of these proteins to functionally complement and transport copper in the corresponding Saccharomyces cerevisiae high-affinity copper transport mutant. The specific expression pattern of the Arabidopsis COPT1–5 mRNA in different tissues was analyzed by RT-PCR. Although all members are ubiquitously expressed, differences in their relative abundance in roots, leaves, stem and flowers have been observed. Moreover, steady-state COPT1 and COPT2 mRNA levels, the members that are most efficacious in complementing the S. cerevisiae high-affinity copper transport mutant, are down-regulated under copper excess, consistent with a role for these proteins in copper transport in Arabidopsis cells.

Arabidopsiscopper transportheterologous complementationRT-PCR64Cu uptake

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Vicente Sancenón
    • 1
  • Sergi Puig
    • 2
  • Helena Mira
    • 1
  • Dennis J. Thiele
    • 2
  • Lola Peñarrubia
    • 1
  1. 1.Departament de Bioquímica i Biologia MolecularUniversitat de ValènciaValènciaSpain
  2. 2.Department of Biological ChemistryUniversity of Michigan Medical SchoolAnn ArborUSA