Polystyrene Microspheres in Tissue-Simulating Phantoms Can Collisionally Quench Fluorescence
10.1023/A:1022318900882 Cite this article as: Vishwanath, K. & Mycek, MA. Journal of Fluorescence (2003) 13: 105. doi:10.1023/A:1022318900882 Abstract
Tissue-simulating phantoms that replicate intrinsic optical properties in a controlled manner are useful for quantitative studies of photon transport in turbid biological media. In such phantoms, polystyrene microspheres are often used to simulate tissue optical scattering. Here, we report that using polystyrene microspheres in fluorescent tissue-simulating phantoms can reduce fluorophore quantum yield via collisional quenching. Fluorescence lifetime spectroscopy was employed to characterize quenching in phantoms consisting of a fluorescein dye and polystyrene microspheres (scattering coefficients μ
∼100-600cm s −1). For this range of tissue-simulating phantoms, analysis using the Stern-Volmer equation revealed that collisional quenching by polystyrene microspheres accounted for a decrease in fluorescence intensity of 6-17% relative to the intrinsic intensity value when no microspheres (quenchers) were present. The intensity decrease from quenching is independent of additional, anticipated losses arising from optical scattering associated with the microspheres. These results suggest that quantitative fluorescence measurements in studies employing such phantoms may be influenced by collisional quenching. Collisional quenching tissue phantoms time-resolved fluorescence lifetime spectroscopy polystyrene microspheres fluorescein dye References
R. Richards-Kortum and E. Sevick-Muraca (1996) Quantitative optical spectroscopy for tissue diagnosis.
Annu. Rev. Phys. Chem.
G. Wagnieres, W. Star, and B. Wilson (1998) In vivo fluorescence spectroscopy and imaging for oncological applications.
B. B. Das, L. Feng, and R. R. Alfano (1997) Time-resolved fluorescence and photon migration studies in biomedical and model random media.
Rep. Progr. Phys.
S. Chandrasekhar (1960)
. Dover, NY
A. J. Welch and M. J. C. van-Gemert (1995)
Optical-Thermal Response of Laser-Irradiated Tissue
. Plenum Press, New York.
A. J. Durkin, S. Jaikumar, and R. Richards-Kortum (1993) Optically dilute, absorbing, and turbid phantoms for fluorescence spectroscopy of homogeneous and inhomogeneous samples.
J. C. Hebden, D. J. Hall, M. Firbank, and D. T. Delpy (1995) Time-resolved optical imaging of a solid tissue-equivalent phantom.
K. Rinzema, L. H. P. Murrer, and W. M. Star (1998) Direct experimental verification of light transport theory in an optical phantom.
J. Optical Soc. Am.
A. E. Cerussi, J. S. Maier, S. Fantini, M. A. Franceschini, W. W. Mantulin, and E. Gratton (1997) Experimental verification of a theory for the time-resolved fluorescence spectroscopy of thick tissues.
J. R. Mourant, T. Fuselier, J. Boyer, T. Johnson, and I. Bigio (1997) Predictions and measurements of scattering and absorption over broad wavelength ranges in tissue phantoms.
A. Sefkow, M. Bree, and M.-A. Mycek (2001) A method for measuring cellular optical absorption and scattering evaluated using dilute cell suspension phantoms.
M. Patterson, B. Chance, and B. Wilson (1989) Time resolved reflectance and transmittance for the non-invasive measurement of tissue optical properties.
E. M. Sevick-Muraca, J. S. Reynolds, T. L. Troy, G. Lopez, and D. Y. Paithankar (1998)
Adv. Optical Biopsy Optical Mammogr.
K. Vishwanath, B. W. Pogue, and M.-A. Mycek (2002) Quantitative fluorescence lifetime spectroscopy in turbid media: Comparison of theoretical, experimental and computational methods.
Phys. Med. Biol.
S. A. Ramakrishna and K. D. Rao (2000) Estimation of light transport parameters in biological media using coherent backscattering.
Pramana J. Phys.
S. J. Madsen, M. S. Patterson, and B. C. Wilson (1992) The use of India ink as an optical absorber in tissue-stimulating phantoms.
Phys. Med. Biol.
C. L. Hutchinson, T. L. Troy, and E. M. Sevick Muraca (1996) Fluorescence-lifetime determination in tissues or other scattering media from measurement of excitation and emission kinetics.
L. Wang, D. Liu, N. He, S. L. Jacques, and S. L. Thomsen (1996) Biological laser action.
J. R. Lakowicz (1999)
Principles of Fluorescence Spectroscopy
. 2nd ed. Kluwer Academic/Plenum, New York.
J. D. Pitts and M.-A. Mycek (2001) Design and development of a rapid acquisition laser-based fluorometer with simultaneous spectral and temporal resolution.
Rev. Sci. Instrum.
W.-F. Cheong, S. Prahl, and S. Welch. (1990) A review of the optical properties of biological tissues.
IEEE J. Quantum Electr.
Google Scholar Copyright information
© Plenum Publishing Corporation 2003