Activated T-Lymphocytes Express Occludin, a Component of Tight Junctions
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
T-lymphocytes routinely traffic from the lymphoid and vascular compartments to the tissues during immune surveillance and inflammatory responses. This egress occurs without compromising endothelial barrier, which is maintained by tight junctions (zonula occludens). We report that T-lymphocytes up-regulate the expression of occludin, a major component of the tight junction in response to stimulation with phorbol ester (PMA) + calcium ionophore, CD3 antibody or T-cell receptor (TCR) antibody. Only activated T-lymphocytes express occludin; this adhesion molecule is nearly absent in resting T-lymphocytes. By immunofluorescence, occludin is seen in lymphocyte aggregates, but does not appear to mediate aggregation since only 50% of the cells in these clusters express occludin. Occludin is expressed between 8 and 24 h following stimulation, and persists for at least 48 h. These data indicate that activated T cells produce occludin which may regulate lymphocyte adhesion and trafficking.
- Allport, J. R., H. Ding, T. Collins, M. E. Gerritsen, and F. W. Luscinskas. 1997. Endothelial dependent mechanisms regulate leukocyte transmigration: a process involving the proteasome and disruption of the vascular endothelial cadherin complex at endothelial cell to cell junctions. J. Exp. Medicine 186(4):517–527. CrossRef
- Anderson, J. M., and C. M. van Italie, 1995. Tight junctions and the molecular basis for regulation of paracellular permeability. Am. J. Physiol. 269 (Gastrointest. Liver Physiol. 32):G467–475.
- Hauzenberger, D., J. Klominek, S. E. Bergstrom, and K. G. Sundqvist, 1995. T-lymphocyte migration: the influence of interactions via adhesion molecules, the T-cell receptor, and cytokines. Crit. Rev. in Immunol. 15(3–4):285–316.
- Dianzani, U. and F. Malavasi, 1995. Lymphocyte adhesion to endothelium. Crit. Rev. in Immunol. 15(2):167–200.
- Devine, L., Lightman, and S. L. Greenwood, 1996. Role of LFA-1, ICAM-1, VLA-4 and VACM-1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro. J. Immunology 88(3):456–462. CrossRef
- Mojcik, C. F., and E. M. Shevach, 1997 Adhesion molecules: A rheumatologic perspective. Arthritis and Rheumatism 40(6):991–1004.
- Furie, M. B., M. C. Tancinco, and C. W. Smith. 1991. Monoclonal antibodies to leukocyte integrins CD11a/CD18 and CD11b/CD18 or intercellular adhesion molecule-1 inhibit chemoattractant-stimulated neutrophil transendothelial migration in vitro. Blood 78(8):2089–2097.
- Madri, J. A., Graesser, D., and Has, T. 1996. The roles of adhesion molecules and proteinases in lymphocyte transendothelial migration. Biochemistry and Cell Biology 74(6):749–757.
- Zocchi, M. R., E. Ferrero, B. E. Leone, P. Rovere, E. Bianchi, E. Toninelli, and R. Pardi. 1996. CD31/PECAM-1 driven chemokine-independent transamigration of human T lymphocytes. Eur. J. of Immunol. 26(4), 759–767.
- Lampugnani, M. G., M. Resnati, M. Raiteri, R. Pigott, A. Pisacane, G. Houen, L. P. Ruco, and E. Dejana. 1992. A novel endothelial specific membrane protein is a marker of cell-cell contacts. J. Cell Biol. 118:1511–1522. CrossRef
- Alexander, J. S., O. Blaschuk, and F. R. Haselton. 1993. An N-cadherin-like protein contributes to solute barrier maintenance in cultured endothelium. J. Cell Physiol. 156:610–618.
- Cepek, K. L., D. L. Rimm, and M. B. Brenner. 1996. Expression of a candidate cadherin in T lymphocytes. Proc. Nat. Acad. Sci. U.S.A., 93:6567–6571. CrossRef
- Huang, A. J., M. B. Furie, S. C. Nicholson, J. Fischbarg, L. S. Leibovitch, and S. C. Silverstein. 1988. Effects of human neutrophil chemotaxis across human endothelial cell monolayers on the permeability of these monolayers to ions and macromolecules. J. of Cell Physiol. 135(3), 355–366.
- Furuse, M., T. Hirase, M. Itoh, A. Nagafuchi, S. Yonemura, Sa, Tsukita, and Sh. Tsukita. 1993. Occludin: a novel integral membrane protein localizing at tight junctions. J. Cell Biol. 123:1777–1788. CrossRef
- Opstelten, D., J. Deenen, J. Rozing, and S. V. Hunt. 1986. B-Lymphocyte associated antigens on terminal deoxynucleotidyl transferase positive cells and pre-B cells in bone marrow of the rat. J. Immunol. 137:76–84.
- Kevil, C. G., L. Walsh, F. S. Laroux, T. Kalogeris, M. B. Grisham and J. S. Alexander, 1997. An Improved, Rapid Northern Protocol. Biochem. Biophys. Res. Comm. 238:277–279. CrossRef
- Takeichi, M. 1988. The Cadherins: Cell-cell adhesion molecules controlling animal morphogenesis. Development 102:639–655.
- Burns, A. R., D. C. Walker, E. S. Brown, L. T. Thurmon, R. A. Bowden, C. R. Keese, S. I. Simon, M. L. Entman, C. W. Smith. 1997. Neutrophil transendothelial migration is independent of tight junctions and occurs preferentially at tricellular corners. J. Immunol. 159:2893–2903.
- Kago, S., S. Ragg, K. A. Rogers, A. Ochi. 1998. Cutting edge: Stimulation of CD28 with B7-2 promotes focal adhesion-like cell contacts where Rho family small g-proteins accumulate in T cells. J. Immuno. 160:24–27.
- Activated T-Lymphocytes Express Occludin, a Component of Tight Junctions
Volume 22, Issue 6 , pp 573-582
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
- Additional Links
- Industry Sectors
- Author Affiliations
- 1. Louisiana State University Medical Center, 1501 Kings Highway, Shreveport, Louisiana, 71130.
- 2. Department of Molecular and Cellular Physiology, Royal Victoria Hospital, 687 Pine Ave West Montreal, Canada, H3A1A1
- 3. Department of Molecular and Cellular Physiology, Royal Victoria Hospital, 687 Pine Ave West Montreal, Canada, H3A1A1
- 4. Department of Urology, Royal Victoria Hospital, 687 Pine Ave West Montreal, Canada, H3A1A1