Journal of Fluorescence

, Volume 13, Issue 1, pp 69–77

Effects of Metallic Silver Particles on Resonance Energy Transfer Between Fluorophores Bound to DNA

  • Joseph R. Lakowicz
  • Józef Kuśba
  • Yibing Shen
  • Joanna Malicka
  • Sabato D'Auria
  • Zygmunt Gryczynski
  • Ignacy Gryczynski
Article

DOI: 10.1023/A:1022306630924

Cite this article as:
Lakowicz, J.R., Kuśba, J., Shen, Y. et al. Journal of Fluorescence (2003) 13: 69. doi:10.1023/A:1022306630924

Abstract

We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4',6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å an to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.

Radiative decay engineeringfluorescence resonance energy transfersilver nanoparticleslabeled DNA

Copyright information

© Plenum Publishing Corporation 2003

Authors and Affiliations

  • Joseph R. Lakowicz
    • 1
  • Józef Kuśba
    • 2
  • Yibing Shen
    • 1
  • Joanna Malicka
    • 2
  • Sabato D'Auria
    • 1
  • Zygmunt Gryczynski
    • 1
  • Ignacy Gryczynski
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of Maryland Baltimore, Center for Fluorescence SpectroscopyBaltimore, Maryland
  2. 2.Faculty of Applied Physics and MathematicsTechnical University of GdańskGdańskPoland