Effects of Metallic Silver Particles on Resonance Energy Transfer Between Fluorophores Bound to DNA
- Cite this article as:
- Lakowicz, J.R., Kuśba, J., Shen, Y. et al. Journal of Fluorescence (2003) 13: 69. doi:10.1023/A:1022306630924
We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4',6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å an to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.