Journal of Applied Phycology

, Volume 14, Issue 5, pp 331–342

Overall assessment of Monodus subterraneus cultivation and EPA production in outdoor helical and bubble column reactors

  • C. Lu
  • F.G. Acién Fernández
  • E. Cañizares Guerrero
  • D.O. Hall
  • E. Molina Grima
Article

DOI: 10.1023/A:1022198428274

Cite this article as:
Lu, C., Acién Fernández, F., Cañizares Guerrero, E. et al. Journal of Applied Phycology (2002) 14: 331. doi:10.1023/A:1022198428274

Abstract

The outdoor production of Monodus subterraneus wasstudied in bubble column and helical reactors, mainly analysing the influenceofdilution rate, air flow rate and solar irradiance on growth rate andbiochemicalcomposition. Photoinhibition and photo-oxidation phenomena were also analysed.The cultures were stressed at high solar irradiance and dissolved oxygenconcentrations. A clear relationship between stress of the cultures and thefluorescence from PSII measurements was observed, the Fv/Fm ratio being lowerinthe helical reactor than in the bubble column. Growth rate and biomassproductivity were both a function of the average irradiance and the Fv/Fmratio;maximum values of 0.040 h−1 and 0.54 gL−1 d−1 were measured. The influenceofphotoinhibition and average irradiance was modelled, the model also fitting theexperimental data reported by another author. The chlorophyll contenthyperbolically decreased, whereas the carotenoid content decreased linearlywiththe average irradiance. The higher the dilution rate the higher the protein andcarbohydrate content of the biomass, and the lower the lipid content. Theeicosapentaenoic acid (EPA) content ranged from 2.3 to 3.2% d.wt, the higherthe dilution rate, the lower EPA content, although the higher the EPAproportion. Maximum EPA productivity was only 9 mg L−1d−1, due to the stress to which the cultures wereexposed.

Bubble columnElemental compositionFluorescence of PSIIHelical reactorMonodusOutdoor reactorsPhotoinhibition

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • C. Lu
    • 1
  • F.G. Acién Fernández
    • 2
  • E. Cañizares Guerrero
    • 2
  • D.O. Hall
    • 1
  • E. Molina Grima
    • 2
  1. 1.Division of Life SciencesKing's College LondonWaterloo LondonUK
  2. 2.Department of Chemical EngineeringUniversity of AlmeríaAlmeríaSpain