Article

Transgenic Research

, Volume 12, Issue 1, pp 115-122

First online:

Plastid Transformation in Lesquerella Fendleri, an Oilseed Brassicacea

  • Marina SkarjinskaiaAffiliated withWaksman Institute, Rutgers, The State University of New Jersey
  • , Zora SvabAffiliated withWaksman Institute, Rutgers, The State University of New Jersey
  • , Pal MaligaAffiliated withWaksman Institute, Rutgers, The State University of New Jersey Email author 

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Abstract

A plastid transformation protocol was developed for Lesquerella fendleri, a species with a high capacity for plant regeneration in tissue culture. Transformation vector pZS391B carried an aadA16gfp marker gene conferring streptomycin–spectinomycin resistance and green fluorescence under UV light. Biolistic transformation of 51 Lesquerella leaf samples, followed by spectinomycin selection, yielded two transplastomic clones. The AAD–GFP fusion protein, the marker gene product, was localized to chloroplasts by confocal laser microscopy. Fertile plants and seed progeny were obtained in line Lf-pZS391B-1. In the 51 samples a large number (108) of spontaneous mutants were identified. In five of the lines spectinomycin resistance was localized to a conserved stem structure by sequencing 16S rRNA genes. Success in L. fendleri, a wild oilseed species, extends plastid transformation beyond Arabidopsis thaliana in the Brassicaceae family.

Lesquerella fendleri plastid transformation rRNA mutations spectinomycin resistance spectinomycin selection