Chromosome Research

, Volume 11, Issue 1, pp 51–56

Cytogenetic and molecular characterization of the MBSAT1 satellite DNA in holokinetic chromosomes of the cabbage moth, Mamestra brassicae (Lepidoptera)

  • Mauro Mandrioli
  • Gian Carlo Manicardi
  • Frantisek Marec
Article

DOI: 10.1023/A:1022058032217

Cite this article as:
Mandrioli, M., Carlo Manicardi, G. & Marec, F. Chromosome Res (2003) 11: 51. doi:10.1023/A:1022058032217

Abstract

Digestion of Mamestra brassicae DNA with DraI produced a prominent fragment of approximately 200 bp and a ladder of electrophoretic bands with molecular weights which are a multiple of 200 bp. Southern blotting revealed that this ladder is composed of DNA fragments that are multimers of the 200-bp DraI band suggesting that DraI isolated a satellite that has been called Mamestra brassicae satellite DNA 1 (MBSAT1). MBSAT1 is the first satellite DNA isolated in Lepidoptera. In-situDraI digestion of chromosome spreads, together with fluorescent in-situ hybridization, showed that MBSAT1 sequences are clustered in heterochromatin of the sex chromosomes, Z and W. MBSAT1 was 234 bp long with an AT content of 60.7%. The curvature–propensity plot suggested a curvature in the MBSAT1 structure.

heterochromatinholocentric chromosomeLepidopteraMamestra brassicaesatellite DNA

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Mauro Mandrioli
    • 1
  • Gian Carlo Manicardi
    • 2
  • Frantisek Marec
    • 3
  1. 1.Dipartimento di Biologia AnimaleUniversità di Modena e Reggio EmiliaModenaItaly
  2. 2.Dipartimento di Scienze AgrarieUniversità degli Studi di Modena e Reggio EmiliaReggio EmilaItaly
  3. 3.Institute of Entomology ASCRCeske BudejoviceCzech Republic