Biotechnology Letters

, Volume 25, Issue 2, pp 173–177

Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources

  • Jingli Xie
  • Li Zhang
  • Qin Ye
  • Qingwei Zhou
  • Li Xin
  • Peng Du
  • Renbao Gan
Article

DOI: 10.1023/A:1021905010021

Cite this article as:
Xie, J., Zhang, L., Ye, Q. et al. Biotechnology Letters (2003) 25: 173. doi:10.1023/A:1021905010021

Abstract

A recombinant strain of Pichia pastoris with a phenotype of MutS was used to produce angiostatin. Due to the low methanol consumption rate of this strain, both methanol and glycerol feedings, that produced oscillation in dissolved O2 concentration, were used during the expression phase to improve cell growth and angiostatin expression. However, enhanced cell growth led to nitrogen limitation that suppressed further production of angiostatin, but addition of ammonia allowed angiostatin concentration to reach 108 mg l−1 after an expression period of 96 h. The ratio of consumed glycerol to methanol of 1.5:1 (w/w) in the expression phase suggested that methanol played an important role in the metabolism of carbon sources.

angiostatindissolved O2glycerol feedingPichia pastoris

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Jingli Xie
    • 1
  • Li Zhang
    • 1
  • Qin Ye
    • 1
  • Qingwei Zhou
    • 2
  • Li Xin
    • 2
  • Peng Du
    • 2
  • Renbao Gan
    • 2
  1. 1.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiChina
  2. 2.the Chinese Academy of ScienceInstitute of Biochemistry and Cell Biology, Shanghai Institute of Biological ScienceShanghaiChina