Conservation Genetics

, Volume 4, Issue 1, pp 95–103

Use of restriction fragment length polymorphisms to identify sea turtle eggs and cooked meats to species

Authors

  • M. Katherine Moore
    • National Oceanic and Atmospheric Administration, National Ocean Service, National Centers for Coastal Ocean ScienceCenter for Coastal Environmental Health and Biomolecular Research at Charleston
  • John A. Bemiss
    • National Oceanic and Atmospheric Administration, National Ocean Service, National Centers for Coastal Ocean ScienceCenter for Coastal Environmental Health and Biomolecular Research at Charleston
  • Susan M. Rice
    • U.S. Fish and Wildlife ServiceEastern Shore of Virginia National Wildlife Refuge
  • Joseph M. Quattro
    • Department of Biological Sciences, Baruch InstituteSchool of the Environment, Program in Marine Science, University of South Carolina
  • Cheryl M. Woodley
    • National Oceanic and Atmospheric Administration, National Ocean Service, National Centers for Coastal Ocean ScienceCenter for Coastal Environmental Health and Biomolecular Research at Charleston
Article

DOI: 10.1023/A:1021881319271

Cite this article as:
Moore, M.K., Bemiss, J.A., Rice, S.M. et al. Conservation Genetics (2003) 4: 95. doi:10.1023/A:1021881319271

Abstract

One of the many threats to sea turtlepopulations is the take of turtles and theireggs for consumption and sale. Improved speciesidentification methods for sea turtle eggs andcooked meats would facilitate prosecution ofthose involved. Fatty acid-based methods toidentify eggs cannot resolve loggerheads andthe two ridley species. Protein-based methodsare not applicable to eggs or cooked meat. Wepresent methods to extract DNA from turtle eggand cooked meat and to produce diagnosticrestriction fragment length polymorphismpatterns in the cytochrome b region of themitochondrial DNA. This method works on DNAfrom any tissue, and provides wildlife lawenforcement another tool to combat illegal takeof endangered species.

forensicmitochondrial DNArestriction fragment length polymorphismsea turtlesspecies identification

Copyright information

© Kluwer Academic Publishers 2003