Cytotechnology

, Volume 38, Issue 1, pp 77–85

Optimisation of protein expression and establishment of the Wave Bioreactor for Baculovirus/insect cell culture

  • Wilfried Weber
  • Eric Weber
  • Sabine Geisse
  • Klaus Memmert
Article

DOI: 10.1023/A:1021102015070

Cite this article as:
Weber, W., Weber, E., Geisse, S. et al. Cytotechnology (2002) 38: 77. doi:10.1023/A:1021102015070

Abstract

As the interest of research is beginning to shift from genomicsto proteomics the number of proteins to be expressed is rapidlyincreasing. To do so, well-established, high-level expressionsystems and rapid, cost-effective production means are needed. For addressing the latter, a novel cultivation system for recombinant cells, the Wave Bioreactor™ has recently becomeavailable. We describe the set-up and the optimisation of parameters essential for successful operation and growth of insect cells to high cell densities in the Wave Bioreactor. According to our experience, the Cellbag™ system comparesvery favorably to conventional cultivation vessels such as bioreactors and roller cultures with respect to simplicity ofoperation and cost. Additionally, we developed a rapid and simple protocol for assessing expression and production conditions for the Baculovirus/insect cell system applicable to many different genes/proteins. Important parameters like MOI,TOI, peak cell density (PCD) and expression levels are determinedin pre-experiments on small scale to achieve optimal expressionof a given protein. These conditions are subsequently transformedand applied to large scale cultures grown in nutrient-supplemented medium in the Wave Bioreactor.

baculovirus/insect cell systemessential parameters for operationexpression optimisationfeeding strategiesWave Bioreactor

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Wilfried Weber
    • 1
  • Eric Weber
    • 1
  • Sabine Geisse
    • 1
  • Klaus Memmert
    • 1
  1. 1.Core Technologies/Biomolecules ProductionNovartis Pharma AG, ResearchBaselSwitzerland
  2. 2.ETH HoenggerbergZürichSwitzerland