Neurochemical Research

, Volume 24, Issue 1, pp 33–36

Stimulation of Immunoreactive Insulin Release by Glucose in Rat Brain Synaptosomes

Authors

  • Maria S. Santos
    • Center for Neurosciences of Coimbra, Department of Zoology. M. S. Santos, Center for Neurosciences of Coimbra, Department of ZoologyUniversity of Coimbra
  • Emilia M. Pereira
    • Faculty of MedicineUniversity of Coimbra
  • Arsélio P. Carvaho
    • Center for Neurosciences of Coimbra, Department of Zoology. M. S. Santos, Center for Neurosciences of Coimbra, Department of ZoologyUniversity of Coimbra
Article

DOI: 10.1023/A:1020971812098

Cite this article as:
Santos, M.S., Pereira, E.M. & Carvaho, A.P. Neurochem Res (1999) 24: 33. doi:10.1023/A:1020971812098

Abstract

The effect of glucose on the release of immunoreactive insulin (IRI) in synaptosomes isolated from rat brain was studied. In the absence of glucose synaptosomes release about 4% (0.77 μIU/mg protein) of total content. Glucose increases significantly the IRI released by synaptosomes. Addition of the glycolytic inhibitor iodoacetic acid (IAA), decreased the glucose-induced release of IRI by about 50%, suggesting that glucose metabolism is involved. The observation that glucose provides a concentration related signal for IRI release indicates that this synaptosomal preparation may be useful as a model for research on the mechanism of insulin release in brain.

Synaptosomesglucosebrain insulininsulin release

Copyright information

© Plenum Publishing Corporation 1999