, Volume 27, Issue 10, pp 1123-1131

Theoretical Consideration of Amplification Strategies

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Abstract

Experimentalists would like to perform large-scale expression analysis on samples drawn from small cell populations and even single cells. Amplification of the source material is required to assay such small samples under all existing array technologies. In this report we consider the very different theoretical properties of linear and PCR-based amplification. A suggestion is made concerning a hybrid amplification strategy incorporating the advantages offered by both approaches.