Neurochemical Research

, Volume 27, Issue 10, pp 1105–1112

cDNA Microarray Analysis of Changes in Gene Expression Induced by Neuronal Hypoxia in Vitro

  • K. Jin
  • X. O. Mao
  • M. W. Eshoo
  • G. del Rio
  • R. Rao
  • D. Chen
  • R. P. Simon
  • D. A. Greenberg
Article

DOI: 10.1023/A:1020913123054

Cite this article as:
Jin, K., Mao, X.O., Eshoo, M.W. et al. Neurochem Res (2002) 27: 1105. doi:10.1023/A:1020913123054
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Abstract

We used cDNA microarray gene expression profiling to characterize the transcriptional response to exposure of cultured mouse cerebral cortical neurons to hypoxia for 24 hr. Of 11,200 genes examined, 1,405 (12.5%) were induced or repressed at least 1.5-fold, whereas 26 known genes were induced and 20 known genes were repressed at least 2.5-fold. The most strongly induced genes included genes coding for endoplasmic reticulum proteins (Ero1L/Giig11, Sac1p, Ddit3/Gadd153), proteins involved in ubiquitination (Arih2, P4hb), proteins induced by hypoxia in non-neuronal systems (Gpi1, Aldo1, Anxa2, Hig1), and proteins that might promote cell death (Gas5, Egr1, Ndr1, Vdac2). These findings reinforce the importance of endoplasmic reticulum-based mechanisms and of protein-ubiquitination pathways in the neuronal response to hypoxia.

Microarrayhypoxiaendoplasmic reticulumubiquitinationhypoxia-inducible factor-1

Copyright information

© Plenum Publishing Corporation 2002

Authors and Affiliations

  • K. Jin
    • 1
  • X. O. Mao
    • 1
  • M. W. Eshoo
    • 1
  • G. del Rio
    • 1
  • R. Rao
    • 1
  • D. Chen
    • 2
  • R. P. Simon
    • 2
  • D. A. Greenberg
    • 1
  1. 1.Buck Institute for Age ResearchNovato
  2. 2.Robert S. Dow Neurobiology LaboratoryLegacy ResearchPortland