Article

Plant Molecular Biology

, Volume 51, Issue 1, pp 71-81

First online:

Salinity stress-tolerant and -sensitive rice (Oryza sativa L.) regulate AKT1-type potassium channel transcripts differently

  • Dortje GolldackAffiliated withLehrstuhl für Stoffwechselphysiologie und Biochemie der Pflanzen, Universität Bielefeld
  • , Francoise QuigleyAffiliated withLaboratoire de Genétique Moléculaire des Plantes, UMR CNRS 5575, Université Joseph Fourier
  • , Christine B. MichalowskiAffiliated withDepartment of Biochemistry, University of Arizona
  • , Uma R. KamasaniAffiliated withDepartment of Biochemistry, University of Arizona
  • , Hans J. BohnertAffiliated withDepartment of Plant SciencesDepartment of Molecular and Cellular Biology, University of Arizona Email author 

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Abstract

In the indica rice (Oryza sativa L.) a cDNA was characterized that encoded OsAKT1 homologous to inward-rectifying potassium channels of the AKT/KAT subfamily. Transcript analysis located OsAKT1 predominantly in roots with low abundance in leaves. Cell-specificity of OsAKT expression was analyzed by in situ hybridizations. In roots, strongest signals were localized to the epidermis and the endodermis, whereas lower transcript levels were detected in cells of the vasculature and the cortex. In leaves, expression was detected in xylem parenchyma, phloem, and mesophyll cells. Transcriptional regulation and cell specificity of OsAKT1 during salt stress was compared in rice lines showing different salinity tolerance. In the salt-tolerant, sodium-excluding varieties Pokkali and BK, OsAKT1 transcripts disappeared from the exodermis in plants treated with 150 mM NaCl for 48 h but OsAKT1 transcription was not repressed in these cells in the salt-sensitive, sodium-accumulating variety IR29. Significantly, all lines were able to maintain potassium levels under sodium stress conditions, while sodium concentrations in the leaves of IR29 increased 5–10-fold relative to the sodium concentration in BK or Pokkali. The divergent, line-dependent and salt-dependent, regulation of this channel does not significantly affect potassium homeostasis under salinity stress. Rather, repression in Pokkali/BK and lack of repression in IR29 correlate with the overall tolerance character of these lines.

in situ hybridization NaCl stress Oryza sativa potassium channel