Cellular and Molecular Neurobiology

, Volume 22, Issue 3, pp 303–314

Gene Transfer of NMDAR1 Subunit Sequences to the Rat CNS Using Herpes Simplex Virus Vectors Interfered with Habituation

  • V. T. Cheli
  • M. F. Adrover
  • C. Blanco
  • E. Rial Verde
  • V. Guyot-Revol
  • R. Vidal
  • E. Martin
  • L. Alché
  • G. Sanchez
  • M. Acerbo
  • A. L. Epstein
  • D. Jerusalinsky
Article

DOI: 10.1023/A:1020720001865

Cite this article as:
Cheli, V.T., Adrover, M.F., Blanco, C. et al. Cell Mol Neurobiol (2002) 22: 303. doi:10.1023/A:1020720001865

Abstract

1. The aim is to study some roles of the hippocampal NMDA receptor, by modifying the expression of the essential NRl subunit, with temporal and spatial restrictions in the central nervous system (CNS) of the rat.

2. Due to their neurotropism and the size of inserts they can accomodate, herpes simplex virus type-l (HSV-1) derived amplicon vectors were used to transfer sequences, either in sense (+) or antisense (-) orientations, of the NRl subunit gene, or of the green fluorescent protein (GFP) gene, into the CNS.

3. Vector expression in cell lines was followed by GFP autofluorescence, immunofluorescence and western blot.

4. The vectors were inoculated into the dorsal hippocampus of adult male Wistar rats, which were evaluated for habituation to an open field, and then, for expression of the transgenes, by autofluorescence and western blot; the expression mainly happened in pyramidal cells of CA1.

5. The animals injected with vectors carrying the NRl(+) transgene showed habituation to the new environment, as also happened with rats injected with vectors carrying only the GFP transgene.

6. In contrast, animals injected with vectors carrying NRl(-) sequence, did not show habituation. This might be retrograde amnesia or disability to record the trace, suggesting that the NRl subunit in the dorsal hippocampus, is involved in habituation to a new environment.

7. HSV-1 derived amplicon vectors appear to be useful tools to modify endogenous gene expression, at a defined period, in restricted regions of the CNS.

herpes simplex vectors NMDA receptors NR1 subunit hippocampus memory learning 

Copyright information

© Plenum Publishing Corporation 2002

Authors and Affiliations

  • V. T. Cheli
    • 1
  • M. F. Adrover
    • 1
  • C. Blanco
    • 2
  • E. Rial Verde
    • 1
  • V. Guyot-Revol
    • 3
  • R. Vidal
    • 2
  • E. Martin
    • 2
  • L. Alché
    • 4
  • G. Sanchez
    • 2
  • M. Acerbo
    • 2
  • A. L. Epstein
    • 3
  • D. Jerusalinsky
    • 1
  1. 1.Institute of Cellular Biology & Neurosciences Professor “Eduardo De Robertis,” School of MedicineUniversity of Buenos AiresBuenos AiresArgentina
  2. 2.Department of Anatomy, School of VeterinaryUniversity of Buenos AiresBuenos AiresArgentina
  3. 3.Center of Cellular and Molecular Genetics, UMR 5534-CNRSUniversity Claude BernardLyonFrance
  4. 4.Department of Virology, School of SciencesUniversity of Buenos AiresBuenos AiresArgentina

Personalised recommendations