Biometals

, Volume 16, Issue 2, pp 349–357

The influence of hypomagnesemia on erythrocyte antioxidant enzyme defence system in mice

  • Andrzej Kuzniar
  • Przemyslaw Mitura
  • Piotr Kurys
  • Stanislawa Szymonik-Lesiuk
  • Boleslaw Florianczyk
  • Marta Stryjecka-Zimmer
Article

DOI: 10.1023/A:1020632505289

Cite this article as:
Kuzniar, A., Mitura, P., Kurys, P. et al. Biometals (2003) 16: 349. doi:10.1023/A:1020632505289

Abstract

The effect of magnesium deficiency on antioxidant defence system was studied in RBC of mice suffering from hypomagnesemia. The animals were kept for 8, 15 and 22 days on magnesium-deficient diet with consequent reduction of magnesium level in plasma by 38% at the first 8 days and by 64% after 22 days of experiment. The activities of the most important antioxidant enzymes, catalase, glutathione peroxidase, superoxide dismutase, glutathione reductase, glutahione S-transferase were assayed in hemolysates. The level of reduced glutathione in erythrocytes was measured as well. Apart from catalase, the activities of antioxidant enzymes were decreasing. The activity of superoxide dismutase decreased gradually during the experiment and on the 15th and 22nd day of experiment was significantly (P<0,05) lowered by 30 and 32% respectively. The catalase activity was increased on each point of the experiment with the peak value up to 149% on 15th day, and by 32% on 22nd day. Glutathione peroxidase activity was insignificantly reduced. The reduction of Glutatione reductase and Glutathione S-transferase activities by 24 and 21%, respectively, were observed after 8 days of the experiment with a further downward tendency. The reduced glutathione was significantly depleted after 8 days by 33% and was kept on that level in the course of the study. These findings support previous reports on the hypomagnesemia – induced alteration in endogenous enzyme antioxidant defences and glutathione redox cycle of mice.

antioxidant enzymes erythrocytes free radicals hypomagnesemia magnesium oxidative stress 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Andrzej Kuzniar
    • 1
  • Przemyslaw Mitura
    • 1
  • Piotr Kurys
    • 1
  • Stanislawa Szymonik-Lesiuk
    • 1
  • Boleslaw Florianczyk
    • 1
  • Marta Stryjecka-Zimmer
    • 1
  1. 1.Poland, Department of Biochemistry and Molecular BiologyMedical University of LublinLublinPoland

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